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Phospho-Chk1 (S280) Recombinant Rabbit Monoclonal Antibody [JE47-66]

Usd: 385

Specification

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Catalog# HA721471

Phospho-Chk1 (S280) Recombinant Rabbit Monoclonal Antibody [JE47-66]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospho-Chk1 (S280) Recombinant Rabbit Monoclonal Antibody [JE47-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser280 of human Chk1.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 54 kDa

Positive Control

293T treated with 100nM Calyculin A for 15 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate.

Conjugation

unconjugated

Clone Number

JE47-66

RRID

AB_3072587

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Checkpoint kinase 1, commonly referred to as Chk1, is a serine/threonine-specific protein kinase that, in humans, is encoded by the CHEK1 gene. Chk1 coordinates the DNA damage response (DDR) and cell cycle checkpoint response. Activation of Chk1 results in the initiation of cell cycle checkpoints, cell cycle arrest, DNA repair and cell death to prevent damaged cells from progressing through the cell cycle. Checkpoint kinases (Chks) are protein kinases that are involved in cell cycle control. Two checkpoint kinase subtypes have been identified, Chk1 and Chk2. Chk1 is a central component of genome surveillance pathways and is a key regulator of the cell cycle and cell survival. Chk1 is required for the initiation of DNA damage checkpoints and has recently been shown to play a role in the normal (unperturbed) cell cycle. Chk1 impacts various stages of the cell cycle including the S phase, G2/M transition and M phase. In addition to mediating cell cycle checkpoints, Chk1 also contributes to DNA repair processes, gene transcription, egg production, embryo development, cellular responses to HIV infection and somatic cell viability.

Background References

1. Mehta KPM et al. CHK1 phosphorylates PRIMPOL to promote replication stress tolerance. Sci Adv. 2022 Apr

2. Klomp JE et al. CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell Rep. 2021 Nov

Subcellular Location

Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

UNIPROT

SwissProt: O14757 Human

SwissProt: O35280 Mouse

SwissProt: Q91ZN7 Rat

Synonyms

C85740 antibody

Cell cycle checkpoint kinase antibody

Checkpoint , S. pombe, homolog of, 1 antibody

Checkpoint kinase 1 antibody

Checkpoint kinase 1 homolog (S. pombe) antibody

CHEK 1 antibody

Chek1 antibody

Chk 1 antibody

Chk1 antibody

CHK1 checkpoint homolog (S. pombe) antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (<a href="/products/HA721471" style="font-weight: bold;text-decoration: underline;">HA721471</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T whole cell lysate<br />Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate<br />Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 4 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721471" style="font-weight: bold;text-decoration: underline;">HA721471</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (HA721471) at 1/1,000 dilution.

    Lane 1: 293T whole cell lysate
    Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate
    Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 4 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721471) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (<a href="/products/HA721471" style="font-weight: bold;text-decoration: underline;">HA721471</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 1 minute 2 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721471" style="font-weight: bold;text-decoration: underline;">HA721471</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (HA721471) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 1 minute 2 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721471) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (<a href="/products/HA721471" style="font-weight: bold;text-decoration: underline;">HA721471</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 whole cell lysate<br />Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate<br />Lane 3: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 4 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721471" style="font-weight: bold;text-decoration: underline;">HA721471</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (HA721471) at 1/1,000 dilution.

    Lane 1: PC-12 whole cell lysate
    Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate
    Lane 3: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 4 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721471) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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