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DXD Recombinant Rabbit Monoclonal Antibody [PSH0-73]

Usd: 385

Specification

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Catalog# HA721474

DXD Recombinant Rabbit Monoclonal Antibody [PSH0-73]

Application

  • ELISA

Reactivity

  • Species independent

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

DXD Recombinant Rabbit Monoclonal Antibody [PSH0-73]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

DXD-OVA

Species Reactivity

Species independent

Validated Applications

ELISA

Conjugation

unconjugated

Clone Number

PSH0-73

RRID

AB_3072590

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA

  • 1:1,000

Target

Function

Trastuzumab deruxtecan, sold under the brand name Enhertu, is an antibody-drug conjugate consisting of the humanized monoclonal antibody trastuzumab (Herceptin) covalently linked to the topoisomerase I inhibitor deruxtecan (a derivative of exatecan). It is licensed for the treatment of breast cancer or gastric or gastroesophageal adenocarcinoma. Trastuzumab binds to and blocks signaling through epidermal growth factor receptor 2 (HER2/neu) on cancers that rely on it for growth. Additionally, once bound to HER2 receptors, the antibody is internalized by the cell, carrying the bound deruxtecan along with it, where it interferes with the cell's ability to make DNA structural changes and replicate its DNA during cell division, leading to DNA damage when the cell attempts to replicate itself, destroying the cell. Trastuzumab deruxtecan is the first approved therapy by the US Food and Drug Administration (FDA) targeted to people with the HER2-low breast cancer subtype subset of HER2-negative breast cancer.

Background References

暂无

Synonyms

DXD antibody

Images

  • Indirect ELISA analysis of Dxd was performed by coating wells of a 96-well plate with 50 µL per well of Dxd-SMCC-CCH diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 50 µL per well of Dxd monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 1 hour at room temperature. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:35,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Indirect ELISA analysis of Dxd was performed by coating wells of a 96-well plate with 50 µL per well of Dxd-SMCC-CCH diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 50 µL per well of Dxd monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 1 hour at room temperature. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:35,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Competitive ELISA analysis of Dxd was performed by coating wells of a 96-well plate with 50 µL per well of Dxd-SMCC-CCH diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 50 µL per well of Dxd monoclonal antibody at concentration of 2 µg/mL with serial diluted Dxd starting from a concentration of 10µg/mL for 1 hour at room temperature. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:30,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Competitive ELISA analysis of Dxd was performed by coating wells of a 96-well plate with 50 µL per well of Dxd-SMCC-CCH diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 50 µL per well of Dxd monoclonal antibody at concentration of 2 µg/mL with serial diluted Dxd starting from a concentration of 10µg/mL for 1 hour at room temperature. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:30,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"