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DDR2 Recombinant Rabbit Monoclonal Antibody [JE31-45]

Usd: 385

Specification

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Catalog# HA721479

DDR2 Recombinant Rabbit Monoclonal Antibody [JE31-45]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

DDR2 Recombinant Rabbit Monoclonal Antibody [JE31-45]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human DDR2 aa 806-855 / 855.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 97 kDa

Positive Control

HeLa cell lysate, SH-SY5Y cell lysate, L929 cell lysate, NIH/3T3 cell lysate, U-2 OS cell lysate, A375 cell lysate, PC-12 cell lysate, rat skeletal muscle tissue.

Conjugation

unconjugated

Clone Number

JE31-45

RRID

AB_3072595

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Discoidin domain-containing receptor 2, also known as CD167b (cluster of differentiation 167b), is a protein that in humans is encoded by the DDR2 gene. Discoidin domain-containing receptor 2 is a receptor tyrosine kinase (RTK). RTKs play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. In the case of DDR2, the ligand is collagen which binds to its extracellular discoidin domain. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. DDR2 has been associated with a number of diseases including fibrosis and cancer.

Background References

1. Lin CC et al. DDR2 upregulation confers ferroptosis susceptibility of recurrent breast tumors through the Hippo pathway. Oncogene. 2021 Mar

2. Elkamhawy A et al. The Journey of DDR1 and DDR2 Kinase Inhibitors as Rising Stars in the Fight Against Cancer. Int J Mol Sci. 2021 Jun

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q16832 Human

SwissProt: Q62371 Mouse

Entrez Gene: 685781 Rat

Synonyms

CD167 antigen-like family member B antibody

CD167b antibody

CD167b antigen antibody

Cell migration inducing protein 20 antibody

DDR 2 antibody

DDR2 antibody

DDR2_HUMAN antibody

Discoidin domain containing receptor 2 antibody

Discoidin domain receptor 2 antibody

Discoidin domain receptor family member 2 antibody

Expand

Images

  • Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (<a href="/products/HA721479" style="font-weight: bold;text-decoration: underline;">HA721479</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (40 µg/Lane)<br />Lane 2: SH-SY5Y cell lysate (40 µg/Lane)<br />Lane 3: L929 cell lysate (40 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (40 µg/Lane)<br />Lane 5: U-2 OS cell lysate (15 µg/Lane)<br />Lane 6: A375 cell lysate (15 µg/Lane)<br />Lane 7: PC-12 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 97 kDa<br />Observed band size: 97 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721479" style="font-weight: bold;text-decoration: underline;">HA721479</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (HA721479) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (40 µg/Lane)
    Lane 2: SH-SY5Y cell lysate (40 µg/Lane)
    Lane 3: L929 cell lysate (40 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (40 µg/Lane)
    Lane 5: U-2 OS cell lysate (15 µg/Lane)
    Lane 6: A375 cell lysate (15 µg/Lane)
    Lane 7: PC-12 cell lysate (10 µg/Lane)

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721479) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-DDR2 antibody (<a href="/products/HA721479" style="font-weight: bold;text-decoration: underline;">HA721479</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721479" style="font-weight: bold;text-decoration: underline;">HA721479</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-DDR2 antibody (HA721479) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721479) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

DDR2 Mouse Monoclonal Antibody [A9H5]

Application: WB

Reactivity: Human,Mouse

Conjugate: unconjugated