E2F6 Recombinant Rabbit Monoclonal Antibody [JE33-69]
Catalog# HA721481
E2F6 Recombinant Rabbit Monoclonal Antibody [JE33-69]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
E2F6 Recombinant Rabbit Monoclonal Antibody [JE33-69]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human E2F6 aa 182-281 / 281.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 32 kDa
Positive Control
Jurkat cell lysate, K-562 cell lysate, mouse brain tissue.
Conjugation
unconjugated
Clone Number
JE33-69
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Background References
1. Dahlet T et al. E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. Nat Commun. 2021 Jun
2. Shao G et al. Circ_0004676 exacerbates triple-negative breast cancer progression through regulation of the miR-377-3p/E2F6/PNO1 axis. Cell Biol Toxicol. 2022 Jul
Subcellular Location
Nucleus.
Synonyms
E2F 6 antibody
E2F binding site modulating activity protein antibody
E2F transcription factor 6 antibody
E2F transcription factor 6 isoform 1 antibody
E2F-6 antibody
E2F6 antibody
E2F6_HUMAN antibody
EMA antibody
MGC111545 antibody
Transcription factor E2F6 antibody
Images
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Western blot analysis of E2F6 on different lysates with Rabbit anti-E2F6 antibody (HA721481) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721481) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-E2F6 antibody (HA721481) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721481) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"