Bmf Recombinant Rabbit Monoclonal Antibody [JE33-57]
Catalog# HA721487
Bmf Recombinant Rabbit Monoclonal Antibody [JE33-57]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Bmf Recombinant Rabbit Monoclonal Antibody [JE33-57]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Bmf aa 1-184 / 184.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
Mouse liver tissue lysate, rat liver tissue lysate, mouse spleen tissue lysate, rat spleen tissue lysate, mouse pancreas tissue lysate, rat pancreas tissue lysate, mouse lung tissue lysate, rat ovary tissue lysate.
Conjugation
unconjugated
Clone Number
JE33-57
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Bcl-2-modifying factor is a protein that in humans is encoded by the BMF gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BMF (gene) has been shown to interact with Bcl-2 and DYNLL2.
Background References
1. Xu F et al. RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression. Int J Biol Sci. 2022 Feb
2. Zhang Y et al. Exosomal microRNA-22-3p alleviates cerebral ischemic injury by modulating KDM6B/BMP2/BMF axis. Stem Cell Res Ther. 2021 Feb
Subcellular Location
Acrosomal vesicle, cytosol, mitochondrial outer membrane, myosin complex, plasma membrane.
Synonyms
Bcl 2 modifying factor antibody
Bcl-2-modifying factor antibody
Bcl2 modifying factor antibody
Bmf antibody
BMF_HUMAN antibody
FLJ00065 antibody
Images
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Western blot analysis of Bmf on different lysates with Rabbit anti-Bmf antibody (HA721487) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Rat spleen tissue lysate
Lane 5: Mouse pancreas tissue lysate
Lane 6: Rat pancreas tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721487) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Bmf on different lysates with Rabbit anti-Bmf antibody (HA721487) at 1/1,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Rat ovary tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 30 kDa
Exposure time: 39 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721487) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"