Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
Catalog# HA721513
Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
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WB
-
Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 106 kDa
Positive Control
Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate.
Conjugation
unconjugated
Clone Number
JE44-61
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
The retinoblastoma protein (protein name abbreviated Rb; gene name abbreviated Rb, RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, inactivating it, and the cell cycle is allowed to progress. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins. Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomavirus, bind and inactivate pRb, this can lead to cancer. The RB gene may have been responsible for the evolution of multicellularity in several lineages of life including animals.
Background References
1. Linn P et al. Targeting RB1 Loss in Cancers. Cancers (Basel). 2021 Jul
2. Yao Y et al. Novel insights into RB1 mutation. Cancer Lett. 2022 Oct
Subcellular Location
Nucleus.
Synonyms
Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
GOS563 exon 17 substitution mutation causes premature stop antibody
OSRC antibody
Osteosarcoma antibody
p105-Rb antibody
P105RB antibody
PP105 antibody
pp110 antibody
PPP1R130 antibody
pRb antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Rb (S780) on different lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution.
Lane 1: Jurkat serum starved for 44 hours whole cell lysate
Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate
Lane 3: Jurkat serum starved for 44 hours then treated with λpp for 1 hour whole cell lysate
Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Phospho-Rb (S780) on NIH/3T3 cell lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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