c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]
Catalog# HA721514
c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]
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WB
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Human
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unconjugated
Overview
Product Name
c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human c-Myb aa 591-640 / 640.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 72 kDa
Positive Control
Jurkat cell lysate, HL-60 cell lysate.
Conjugation
unconjugated
Clone Number
JE40-07
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Myb genes are part of a large gene family of transcription factors found in animals and plants. In humans, it includes Myb proto-oncogene like 1 and Myb-related protein B in addition to MYB proper. Members of the extended SANT/Myb family also include the SANT domain and other similar all-helical homeobox-like domains. The Myb gene family is named after the eponymous gene in Avian myeloblastosis virus. The viral Myb (v-Myb, P01104) recognizes the sequence 5'-YAACKG-3'. It causes myeloblastosis (myeloid leukemia) in chickens. Compared to the normal animal cellular Myb (c-myb), v-myb contains deletions in the C-terminal regulatory domain, leading to aberrant activation of other oncogenes.
Background References
1. Říhová K et al. Transcription factor c-Myb: novel prognostic factor in osteosarcoma. Clin Exp Metastasis. 2022 Apr
2. Bu C et al. c-Myb protects cochlear hair cells from cisplatin-induced damage via the PI3K/Akt signaling pathway. Cell Death Discov. 2022 Feb
Subcellular Location
Nucleus.
UNIPROT
Synonyms
C myb antibody
c myb protein (140 AA) antibody
c-myb protein (140 AA) antibody
c-myb_CDS antibody
c-myb10A_CDS antibody
c-myb13A_CDS antibody
c-myb14A_CDS antibody
c-myb8B_CDS antibody
Cmyb antibody
efg antibody
ExpandImages
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Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA721514) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HL-60 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721514) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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