phospho-Acetyl Coenzyme A Carboxylase (S79) Recombinant Rabbit Monoclonal Antibody [JE55-54] (HA721516)
Catalog# HA721516
Rabbit Monoclonal to phospho-Acetyl Coenzyme A Carboxylase (S79)
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-Acetyl Coenzyme A Carboxylase (S79) on different lysates with Rabbit anti-Phospho-Acetyl Coenzyme A Carboxylase (S79) antibody (HA721516) at 1/1,000 dilution.
Lane 1: SH-SY5Y whole cell lysate
Lane 2: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes whole cell lysate
Lane 3: SH-SY5Y treated with λpp for 1 hour whole cell lysate
Lane 4: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 277 kDa
Observed band size: 277 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y whole cell lysate
Lane 2: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes whole cell lysate
Lane 3: SH-SY5Y treated with λpp for 1 hour whole cell lysate
Lane 4: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 277 kDa
Observed band size: 277 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
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