SEC62 Recombinant Rabbit Monoclonal Antibody [PSH0-91]
Catalog# HA721526
SEC62 Recombinant Rabbit Monoclonal Antibody [PSH0-91]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SEC62 Recombinant Rabbit Monoclonal Antibody [PSH0-91]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SEC62 aa 71-120 / 399.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7 cell lysate, Daudi cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH0-91
RRID
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Translocation protein SEC62 is a protein that in humans is encoded by the SEC62 gene. The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.
Background References
1. Su S et al. Sec62 promotes gastric cancer metastasis through mediating UPR-induced autophagy activation. Cell Mol Life Sci. 2022 Feb
2. Liu X et al. Sec62 promotes stemness and chemoresistance of human colorectal cancer through activating Wnt/β-catenin pathway. J Exp Clin Cancer Res. 2021 Apr
Subcellular Location
Endoplasmic reticulum membrane.
Synonyms
Dtrp1 antibody
FLJ32803 antibody
hTP-1 antibody
HTP1 antibody
Membrane protein SEC62, S.cerevisiae, homolog of antibody
OTTHUMP00000213390 antibody
sec62 antibody
SEC62 homolog (S. cerevisiae) antibody
SEC62_HUMAN antibody
TLOC1 antibody
ExpandImages
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Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA721526) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (20 µg/Lane)
Lane 8: Daudi cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: Mouse liver tissue lysate (40 µg/Lane)
Lane 11: Mouse brain tissue lysate (40 µg/Lane)
Lane 12: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"