Biotin Conjugated HIV1 p24 Recombinant Rabbit Monoclonal Antibody [PS01-31] - Detector
Overview
Product Name
Biotin Conjugated HIV1 p24 Recombinant Rabbit Monoclonal Antibody [PS01-31] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within HIV1 p24 aa 133-363 (HA210738).
Species Reactivity
HIV1
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombanint HIV1 p24 protein (HA210738).
Conjugation
Biotin
Clone Number
PS01-31
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-35] to HIV1 p24 (Capture) (HA721527) and Recombinant HIV1 p24 protein (HA210738) as the standard. The reference range value is 39.06-5,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
One distinctive HIV antigen is a viral protein called p24, a structural protein that makes up most of the HIV viral core, or 'capsid'. There are approximately 2000 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24. The onset of symptoms of AIDS correlates with a reduction in the number of CD4+ T-cells and increased levels of virus and p24 in the blood. It is a component of the gag polyprotein. High levels of p24 are present in the blood serum of newly infected individuals during the short period between infection and seroconversion, making p24 antigen assays useful in diagnosing primary HIV infection. Antibodies to p24 are produced during seroconversion, rendering p24 antigen undetectable after seroconversion in most cases. Therefore, p24 antigen assays are not reliable for diagnosing HIV infection after its very earliest stages. Fourth-generation HIV immunoassays detect viral p24 protein in the blood (as well as patient antibodies against the virus). Previous generation tests relied on detecting patient antibodies alone; it takes about 3–4 weeks for the earliest antibodies to be detected. The p24 protein can be detected in patient blood as early as 2 weeks after HIV infection, further reducing the window period necessary to accurately detect the HIV status of the patient.
Background References
1. Chukkapalli V. et al. Evidence in support of RNA-mediated inhibition of phosphatidylserine-dependent HIV-1 Gag membrane binding in cells. J. Virol. 87:7155-7159(2013).
2. Saad J.S. et al. Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly. Proc. Natl. Acad. Sci. U.S.A. 103:11364-11369(2006).
Subcellular Location
Virion.
UNIPROT
Synonyms
CA antibody
Capsid protein p24 antibody
HIV-1 Gag p24 antibody
HIV1gp1 antibody
Human immunodeficiency virus 1 antibody
Human immunodeficiency virus type 1 p24 antibody
Images
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![Sandwich ELISA analysis of HIV1 p24 protein matched pair antibodies<br /><br />Capture: <a href="/products/HA721527" style="font-weight: bold;text-decoration: underline;">HA721527</a>, HIV1 p24 Rabbit mAb [PS01-35]<br />Detector: <a href="/products/HA721528" style="font-weight: bold;text-decoration: underline;">HA721528</a>, HIV1 p24 Rabbit mAb [PS01-31]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721527" style="font-weight: bold;text-decoration: underline;">HA721527</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant HIV1 p24 protein (<a href="/products/HA210738" style="font-weight: bold;text-decoration: underline;">HA210738</a>) starting from 5,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA721528" style="font-weight: bold;text-decoration: underline;">HA721528</a>, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721529_1.jpg?v=20260918230321)
Sandwich ELISA analysis of HIV1 p24 protein matched pair antibodies
Capture: HA721527, HIV1 p24 Rabbit mAb [PS01-35]
Detector: HA721528, HIV1 p24 Rabbit mAb [PS01-31]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721527) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant HIV1 p24 protein (HA210738) starting from 5,000 pg/mL to 0 pg/mL and detect antibody (HA721528, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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