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FUNDC1 Recombinant Rabbit Monoclonal Antibody [PSH0-92]

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Specification

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Catalog# HA721530

FUNDC1 Recombinant Rabbit Monoclonal Antibody [PSH0-92]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FUNDC1 Recombinant Rabbit Monoclonal Antibody [PSH0-92]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within N terminal human FUNDC1.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 17 kDa

Positive Control

293T over-expressed FUNDC1 cell lysate, HepG2 cell lysates, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH0-92

RRID

AB_3072646

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Rat
Not Recommended
Tested Tested
Pig
Tested
Monkey
Tested
Mouse
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50

Target

Function

FUN14 domain containing 1 is a protein that in humans is encoded by the FUNDC1 gene. Model organisms have been used in the study of FUNDC1 function. A conditional knockout mouse line, called Fundc1 was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty two tests were carried out on mutant mice, however no significant abnormalities were observed.

Background References

1. Cai C et al. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway. Redox Biol. 2022 Jun

2. Pei Z et al. FUNDC1 insufficiency sensitizes high fat diet intake-induced cardiac remodeling and contractile anomaly through ACSL4-mediated ferroptosis. Metabolism. 2021 Sep

Subcellular Location

Mitochondrion outer membrane.

UNIPROT

SwissProt: Q8IVP5 Human

SwissProt: Q5BJS4 Rat

Synonyms

FUN14 domain containing 1 antibody

FUN14 domain containing protein 1 antibody

FUN14 domain-containing protein 1 antibody

FUND1_HUMAN antibody

fundc1 antibody

Images

  • Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody (<a href="/products/HA721530" style="font-weight: bold;text-decoration: underline;">HA721530</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T over-expressed FUNDC1 cell lysate<br />Lane 2: 293T control cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 17 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721530" style="font-weight: bold;text-decoration: underline;">HA721530</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FUNDC1 on different lysates with Rabbit anti-FUNDC1 antibody (HA721530) at 1/1,000 dilution.

    Lane 1: 293T over-expressed FUNDC1 cell lysate
    Lane 2: 293T control cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721530) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of FUNDC1 on HepG2 cell lysates with Rabbit anti-FUNDC1 antibody (<a href="/products/HA721530" style="font-weight: bold;text-decoration: underline;">HA721530</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 17 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721530" style="font-weight: bold;text-decoration: underline;">HA721530</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FUNDC1 on HepG2 cell lysates with Rabbit anti-FUNDC1 antibody (HA721530) at 1/1,000 dilution.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721530) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-FUNDC1 antibody (<a href="/products/HA721530" style="font-weight: bold;text-decoration: underline;">HA721530</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721530" style="font-weight: bold;text-decoration: underline;">HA721530</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-FUNDC1 antibody (HA721530) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721530) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation