Laminin 2 alpha Recombinant Rabbit Monoclonal Antibody [PSH0-94]
Catalog# HA721532
Laminin 2 alpha Recombinant Rabbit Monoclonal Antibody [PSH0-94]
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Laminin 2 alpha Recombinant Rabbit Monoclonal Antibody [PSH0-94]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Laminin 2 alpha 1,151-1,400 / 3,122.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 344 kDa
Positive Control
Mouse heart tissue lysate, rat heart tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human heart tissue, mouse heart tissue.
Conjugation
unconjugated
Clone Number
PSH0-94
RRID
Reactivity Data
| WB | IHC-P | IF-Tissue | |
|---|---|---|---|
| Human |
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| Mouse |
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|
|
| Rat |
|
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
-
IHC-P
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1:1,000
-
IF-Tissue
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1:1,000
Target
Function
Laminin subunit alpha-2 is a protein that in humans is encoded by the LAMA2 gene. Laminin, an extracellular matrix protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.
Background References
1. Sarkozy A et al. LAMA2-Related Dystrophies: Clinical Phenotypes, Disease Biomarkers, and Clinical Trial Readiness. Front Mol Neurosci. 2020 Aug
2. Liang J et al. Mex3a interacts with LAMA2 to promote lung adenocarcinoma metastasis via PI3K/AKT pathway. Cell Death Dis. 2020 Aug
Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane.
Synonyms
LAMA 2 antibody
LAMA2 antibody
LAMA2_HUMAN antibody
Laminin alpha 2 (merosin congenital muscular dystrophy) antibody
Laminin alpha 2 antibody
Laminin alpha 2 chain antibody
Laminin alpha 2 subunit antibody
Laminin M antibody
Laminin M chain antibody
Laminin subunit alpha-2 antibody
ExpandImages
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Western blot analysis of Laminin 2 alpha on different lysates with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lane 4: Rat skeletal muscle tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 344 kDa
Observed band size: 344 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721532) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Laminin 2 alpha with Rabbit anti-Laminin 2 alpha antibody (HA721532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721532, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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