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TFF3/Trefoil Factor 3 Recombinant Rabbit Monoclonal Antibody [JE32-01]

Usd: 385

Specification

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Catalog# HA721541

TFF3/Trefoil Factor 3 Recombinant Rabbit Monoclonal Antibody [JE32-01]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TFF3/Trefoil Factor 3 Recombinant Rabbit Monoclonal Antibody [JE32-01]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Trefoil Factor 3 aa 31-80 / 80.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 8 kDa

Positive Control

Human small intestine tissue lysates, human small intestine tissue, HT-29.

Conjugation

unconjugated

Clone Number

JE32-01

RRID

AB_3072657

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfide bonds. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are diverse, including protection of the mucosa, thickening of the mucus, and increasing epithelial healing rates. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Background References

1. Lin Z et al. Trefoil factor 3: New highlights in chronic kidney disease research. Cell Signal. 2022 Sep

2. Yang Y et al. Pathological and therapeutic roles of bioactive peptide trefoil factor 3 in diverse diseases: recent progress and perspective. Cell Death Dis. 2022 Jan

Subcellular Location

Secreted > extracellular space > extracellular matrix > apical lamina.

UNIPROT

SwissProt: Q07654 Human

Synonyms

hITF antibody

hP1.B antibody

Intestinal trefoil factor antibody

ITF antibody

mITF antibody

OTTMUSP00000021729 antibody

P1B antibody

Polypeptide P1.B antibody

TFF3 antibody

TFF3_HUMAN antibody

Expand

Images

  • Western blot analysis of TFF3/Trefoil Factor 3 on human small intestine tissue lysates with Rabbit anti-TFF3/Trefoil Factor 3 antibody (<a href="/products/HA721541" style="font-weight: bold;text-decoration: underline;">HA721541</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 8 kDa<br />Observed band size: 11 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721541" style="font-weight: bold;text-decoration: underline;">HA721541</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TFF3/Trefoil Factor 3 on human small intestine tissue lysates with Rabbit anti-TFF3/Trefoil Factor 3 antibody (HA721541) at 1/1,000 dilution.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 8 kDa
    Observed band size: 11 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721541) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-TFF3/Trefoil Factor 3 antibody (<a href="/products/HA721541" style="font-weight: bold;text-decoration: underline;">HA721541</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721541" style="font-weight: bold;text-decoration: underline;">HA721541</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-TFF3/Trefoil Factor 3 antibody (HA721541) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721541) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-TFF3/Trefoil Factor 3 antibody (<a href="/products/HA721541" style="font-weight: bold;text-decoration: underline;">HA721541</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721541" style="font-weight: bold;text-decoration: underline;">HA721541</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-TFF3/Trefoil Factor 3 antibody (HA721541) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721541) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of HT-29 cells labeling TFF3/Trefoil Factor 3.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721541" style="font-weight: bold;text-decoration: underline;">HA721541</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HT-29 cells labeling TFF3/Trefoil Factor 3.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721541, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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Citation