FOLR1 Recombinant Rabbit Monoclonal Antibody [JE31-40]
Catalog# HA721551
FOLR1 Recombinant Rabbit Monoclonal Antibody [JE31-40]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
FOLR1 Recombinant Rabbit Monoclonal Antibody [JE31-40]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human FOLR1 aa 21-70 / 257.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 30 kDa
Positive Control
HeLa cell lysate, JAR cell lysate, rat testis tissue lysate, A549 cell lysate, SK-OV-3 cell lysate, rat brain tissue lysates.
Conjugation
unconjugated
Clone Number
JE31-40
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Folate receptor 1 (Folate receptor alpha, FOLR1) is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. Members of this family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This receptor is responsible for binding to folic acid and its derivatives, which becomes crucial during fetal development. By adding folate supplementation during pregnancy, neural tube defects in the fetus are prevented. Folate derivatives are necessary for important metabolic processes such as DNA, protein and lipid methylation. More importantly, folate plays a major role in DNA replication and cell division, which are common characteristics of rapid growth. Even though it is unclear how folate affects neural tube formation, scientists are certain that without appropriate folate levels, neural tube defects can develop through human and mice studies. Neural tube defects refer to the improper development of the neural tube by not being sealed correctly. This results in exencephaly or spina bifida, both nervous system abnormalities. This gene is composed of 7 exons; exons 1 through 4 encode the 5' UTR and exons 4 through 7 encode the open reading frame. Due to the presence of 2 promoters, multiple transcription start sites, and alternative splicing of exons, several transcript variants are derived from this gene. These variants differ in the lengths of 5' and 3' UTR, but they encode an identical amino acid sequence.
Background References
1. Nawaz FZ et al. Emerging roles for folate receptor FOLR1 in signaling and cancer. Trends Endocrinol Metab. 2022 Mar
2. Liang Z et al. Tandem CAR-T cells targeting FOLR1 and MSLN enhance the antitumor effects in ovarian cancer. Int J Biol Sci. 2021 Oct
Subcellular Location
Cell membrane
Synonyms
adult antibody
Adult folate binding protein antibody
Adult folate-binding protein antibody
FBP antibody
Folate Binding Protein antibody
Folate Receptor 1 Adult antibody
Folate receptor 1 antibody
Folate Receptor 1 Precursor antibody
Folate receptor adult antibody
Folate receptor alpha antibody
ExpandImages
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Western blot analysis of FOLR1 on different lysates with Rabbit anti-FOLR1 antibody (HA721551) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: JAR cell lysate (20 µg/Lane)
Lane 3: Rat testis tissue lysate (40 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: SK-OV-3 cell lysate (20 µg/Lane)
Predicted band size: 30 kDa
Observed band size: 37~70 kDa
Exposure time: 2 minutes 37 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721551) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of FOLR1 on rat brain tissue lysates with Rabbit anti-FOLR1 antibody (HA721551) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 37 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721551) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"