MCM4 Recombinant Rabbit Monoclonal Antibody [JE38-76]
Catalog# HA721557
MCM4 Recombinant Rabbit Monoclonal Antibody [JE38-76]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
MCM4 Recombinant Rabbit Monoclonal Antibody [JE38-76]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MCM4 aa 800-863.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 97 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, HL-60 cell lysate, human tonsil tissue.
Conjugation
unconjugated
Clone Number
JE38-76
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:1,000
Target
Function
DNA replication licensing factor MCM4 is a protein that in humans is encoded by the MCM4 gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Background References
1. Zhou H et al. Identification of MCM4 as a Prognostic Marker of Hepatocellular Carcinoma. Biomed Res Int. 2021 Dec
2. Xu Y et al. MCM4 in human hepatocellular carcinoma: a potent prognostic factor associated with cell proliferation. Biosci Trends. 2021 May
Subcellular Location
Nucleus, Chromosome.
UNIPROT
Synonyms
19G antibody
AI325074 antibody
AU045576 antibody
CDC 21 antibody
CDC 54 antibody
CDC21 antibody
CDC21 homolog antibody
CDC21, S. pombe, homolog of antibody
CDC54 antibody
Cell division cycle 21, S. pombe, homolog of antibody
ExpandImages
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Western blot analysis of MCM4 on different lysates with Rabbit anti-MCM4 antibody (HA721557) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: HL-60 cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721557) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MCM4 antibody (HA721557) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721557) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"