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FOXL2 Recombinant Rabbit Monoclonal Antibody [JE38-52]

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Specification

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Catalog# HA721558

FOXL2 Recombinant Rabbit Monoclonal Antibody [JE38-52]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FOXL2 Recombinant Rabbit Monoclonal Antibody [JE38-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human FOXL2 aa 1-50 / 376.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 39 kDa

Positive Control

THP-1 cell lysate, K-562 cell lysate, human ovary tissue, mouse ovary tissue.

Conjugation

unconjugated

Clone Number

JE38-52

RRID

AB_3072674

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.

Background References

1. Du H et al. FOXL2 regulates the expression of the Col4a1 collagen gene in chicken granulosa cells. Mol Reprod Dev. 2022 Feb

2. Li R et al. Increased FOXL2 expression alters uterine structures and functions†. Biol Reprod. 2020 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P58012 Human

SwissProt: O88470 Mouse

Synonyms

Blepharophimosis antibody

Blepharophimosis epicanthus inversus and ptosis 1 antibody

Blepharophimosis epicanthus inversus and ptosis antibody

BPES 1 antibody

BPES antibody

BPES1 antibody

Epicanthus inversus and ptosis 1 antibody

Forkhead box L2 antibody

Forkhead box protein L2 antibody

Forkhead transcription factor FOXL2 antibody

Expand

Images

  • Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody (<a href="/products/HA721558" style="font-weight: bold;text-decoration: underline;">HA721558</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: K-562 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 39 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721558" style="font-weight: bold;text-decoration: underline;">HA721558</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody (HA721558) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: K-562 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 39 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721558) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-FOXL2 antibody (<a href="/products/HA721558" style="font-weight: bold;text-decoration: underline;">HA721558</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721558" style="font-weight: bold;text-decoration: underline;">HA721558</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-FOXL2 antibody (HA721558) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721558) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-FOXL2 antibody (<a href="/products/HA721558" style="font-weight: bold;text-decoration: underline;">HA721558</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721558" style="font-weight: bold;text-decoration: underline;">HA721558</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-FOXL2 antibody (HA721558) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721558) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation