CRMP2 Recombinant Rabbit Monoclonal Antibody [JE31-53]
Catalog# HA721560
CRMP2 Recombinant Rabbit Monoclonal Antibody [JE31-53]
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CRMP2 Recombinant Rabbit Monoclonal Antibody [JE31-53]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CRMP2 aa 473-572 / 572.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 62 kDa
Positive Control
U-87 MG cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y.
Conjugation
unconjugated
Clone Number
JE31-53
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
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FC
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1:500-1:1,000
Target
Function
Members of the CRMP family were discovered independently in different species by several groups working in parallel. Among the five members of the family, CRMP-2 was first identified in 1995. Group of researchers led by Goshima found out that CRMP-2 played a role in the transduction of the extracellular Semaphorin 3A (Sema3A), an inhibitory protein for axonal guidance in chick dorsal root ganglion (DRG). The protein was first named as CRMP-62 having a relative molecular mass of 62 kDa and later referred as CRMP-2. Concurrently, a 64 kDa protein named as TOAD-64 for Turned On After Division, was shown to increase significantly during the development of the cortex of the brain. The cDNA sequence of TOAD-64 corresponded to that of rat CRMP-2. In 1996, mouse CRMP-4, often referred to as Ulip for Unc-33 like phosphoprotein, was discovered by Byk and colleagues, using a rabbit polyclonal antiserum which recognized a 64 kDa mouse brain specific phosphoprotein. In the same year, several other studies cloned CRMPs-1-4 in rat and dihydropyrimidinase (DHPase) homologous sequence of CRMPs-1, -2, and -4 in human fetal brain. Finally, in 2000, CRMP-5 was discovered using two-hybrid screenings of brain libraries or purification from a proteic complex. In following researches, CRMPs were studied as target antigens for autoantibodies in various autoimmune neurodegenerative disorders.
Background References
1. Charrier E.; Reibel S.; Rogemond V.; Aguera M.; Thomasset N.; Honnorat J. (August 2003). "Collapsin response mediator proteins (CRMPs) - Involvement in nervous system development and adult neurodegenerative disorders". Molecular Neurobiology. 28 (1): 51–63.
2. Hou ST, Jiang SX, Smith RA (2008). Permissive and repulsive cues and signaling pathways of axonal outgrowth and regeneration. International Review of Cell and Molecular Biology. Vol. 267. pp. 125–181.
Subcellular Location
Cytoplasm. Cytoskeleton.
Synonyms
Collapsin response mediator protein 2 antibody
Collapsin response mediator protein antibody
Collapsin response mediator protein hCRMP 2 antibody
CRAM antibody
CRMP 2 antibody
CRMP-2 antibody
CRMP2 antibody
DHPRP 2 antibody
DHPRP2 antibody
Dihydropyrimidinase 2 antibody
ExpandImages
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Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (HA721560) at 1/1,000 dilution.
Lane 1: U-87 MG cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (10 µg/Lane)
Lane 3: Neuro-2a cell lysate (15 µg/Lane)
Lane 4: PC-12 cell lysate (10 µg/Lane)
Lane 5: Mouse brain tissue lysate (10 µg/Lane)
Lane 6: Rat brain tissue lysate (10 µg/Lane)
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721560) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SH-SY5Y cells labeling CRMP2 with Rabbit anti-CRMP2 antibody (HA721560) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRMP2 antibody (HA721560) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of SH-SY5Y cells labeling CRMP2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721560, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"