Phospholipase C beta 3/PLCB3 Recombinant Rabbit Monoclonal Antibody [JE39-14]
Catalog# HA721561
Phospholipase C beta 3/PLCB3 Recombinant Rabbit Monoclonal Antibody [JE39-14]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Phospholipase C beta 3/PLCB3 Recombinant Rabbit Monoclonal Antibody [JE39-14]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Phospholipase C beta 3/PLCB3 aa 1,161-1,210 / 1,234.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 139 kDa
Positive Control
K-562 cell lysate, MCF7 cell lysate, HeLa cell lysate, HCT 116 cell lysate, SH-SY5Y cell lysate, human small intestine tissue.
Conjugation
unconjugated
Clone Number
JE39-14
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IHC-P
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1:2,000
Target
Function
1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3 is an enzyme that in humans is encoded by the PLCB3 gene. The gene codes for the enzyme phospholipase C β3. The enzyme catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11, as well as G-beta gamma subunits. PLCB3 has been shown to interact with Sodium-hydrogen exchange regulatory cofactor 2.
Background References
1. Zhang X et al. Cetuximab inhibits colorectal cancer development through inactivating the Wnt/beta-catenin pathway and modulating PLCB3 expression. Sci Rep. 2024 May
Subcellular Location
Cytoplasm. Membrane. Nucleus.
UNIPROT
Synonyms
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
1-phosphatidylinositol-4 antibody
5-bisphosphate phosphodiesterase beta-3 antibody
Phosphoinositide phospholipase C antibody
Phosphoinositide phospholipase C-beta-3 antibody
Phospholipase C beta 3 antibody
Phospholipase C beta 3 (phosphatidylinositol specific) antibody
Phospholipase C-beta-3 antibody
PLC beta 3 antibody
PLC-beta-3 antibody
ExpandImages
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Western blot analysis of Phospholipase C beta 3/PLCB3 on different lysates with Rabbit anti-Phospholipase C beta 3/PLCB3 antibody (HA721561) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Predicted band size: 139 kDa
Observed band size: 150 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721561) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Phospholipase C beta 3/PLCB3 antibody (HA721561) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721561) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"