Yes1 Recombinant Rabbit Monoclonal Antibody [JE37-26]
Catalog# HA721563
Yes1 Recombinant Rabbit Monoclonal Antibody [JE37-26]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
Yes1 Recombinant Rabbit Monoclonal Antibody [JE37-26]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Yes1 aa 480 to the C-terminus.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 61 kDa
Positive Control
HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, A549 cell lysate, human kidney tissue, mouse kidney tissue.
Conjugation
unconjugated
Clone Number
JE37-26
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
Target
Function
Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity.
Background References
1. Sprowl J.A., Ong S.S., Gibson A.A., Hu S., Du G., Lin W. A phosphotyrosine switch regulates organic cation transporters. Nat. Commun. 7:10880-10880 (2016).
2. Sprowl J.A., Ong S.S., Gibson A.A., Hu S., Du G., Lin W., Li L., Bharill S., Ness R.A., Pabla N. A phosphotyrosine switch regulates organic cation transporters. Nat. Commun. 7:10880-10880 (2016).
Subcellular Location
Cytoplasm > cytosol.
Synonyms
c Yes antibody
Cellular Yes 1 protein antibody
Cellular Yes1 protein antibody
HsT441 antibody
P61 YES antibody
p61-Yes antibody
Proto oncogene tyrosine protein kinase YES antibody
Proto-oncogene c-Yes antibody
Tyrosine-protein kinase Yes antibody
v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody
ExpandImages
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Western blot analysis of Yes1 on different lysates with Rabbit anti-Yes1 antibody (HA721563) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721563) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Yes1 antibody (HA721563) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721563) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Yes1 antibody (HA721563) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721563) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"