MBNL1 Recombinant Rabbit Monoclonal Antibody [JE38-34]
Catalog# HA721564
MBNL1 Recombinant Rabbit Monoclonal Antibody [JE38-34]
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WB
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Human
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Mouse
Predicted species support after-sales service
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unconjugated
Overview
Product Name
MBNL1 Recombinant Rabbit Monoclonal Antibody [JE38-34]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MBNL1 aa 339-388 / 388.
Species Reactivity
Human (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
Jurkat cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, A549 cell lysate, HeLa cell lysate.
Conjugation
unconjugated
Clone Number
JE38-34
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities.
Background References
1. Warf M.B., Diegel J.V., von Hippel P.H., Berglund J.A. The protein factors MBNL1 and U2AF65 bind alternative RNA structures to regulate splicing. Proc. Natl. Acad. Sci. U.S.A. 106:9203-9208 (2009).
2. Onishi H., Kino Y., Morita T., Futai E., Sasagawa N., Ishiura S. MBNL1 associates with YB-1 in cytoplasmic stress granules. J. Neurosci. Res. 86:1994-2002 (2008).
Subcellular Location
Nucleus. Cytoplasm.
UNIPROT
Synonyms
EXP antibody
EXP35 antibody
EXP40 antibody
EXP42 antibody
KIAA0428 antibody
MBNL antibody
MBNL protein antibody
MBNL1 antibody
MBNL1_HUMAN antibody
Muscleblind 41kD isoform antibody
ExpandImages
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Western blot analysis of MBNL1 on different lysates with Rabbit anti-MBNL1 antibody (HA721564) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: U-2 OS cell lysate
Lane 5: A549 cell lysate
Lane 6: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 37/42 kDa
Exposure time: 2 minutes 33 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721564) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"