FGFR4 Recombinant Rabbit Monoclonal Antibody [JE59-96]
Catalog# HA721578
FGFR4 Recombinant Rabbit Monoclonal Antibody [JE59-96]
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WB
-
FC
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Human
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unconjugated
Overview
Product Name
FGFR4 Recombinant Rabbit Monoclonal Antibody [JE59-96]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human FGFR4 aa 753-802 / 802.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 88 kDa
Positive Control
HepG2 cell lysate, Huh7 cell lysate, SW480 cell lysate, Raji cell lysate, PANC-1 cell lysate, MCF7 cell lysate, Caco-2.
Conjugation
unconjugated
Clone Number
JE59-96
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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FC
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1:500-1:1,000
Target
Function
Fibroblast growth factor receptor 4 is a protein that in humans is encoded by the FGFR4 gene. FGFR4 has also been designated as CD334 (cluster of differentiation 334). The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. In a meta-analisis study, the functional polymorphism Gly388Arg (rs351855) of FGFR4 was observed to be significantly associated with nodal involvement and overall survival in patients with different types of cancer.
Background References
1. Levine KM et al. FGFR4: A promising therapeutic target for breast cancer and other solid tumors. Pharmacol Ther. 2020 Oct
2. Zou Y et al. N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer. Nat Commun. 2022 May
Subcellular Location
Cell membrane, Endosome, Endoplasmic reticulum; Secreted.
UNIPROT
Synonyms
CD 334 antibody
CD334 antibody
CD334 antigen antibody
fc13h 10 antibody
fc13h10 antibody
Fgfr 4 antibody
FGFR-4 antibody
Fgfr4 antibody
FGFR4_HUMAN antibody
Fibroblast growth factor receptor 4 antibody
ExpandImages
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Western blot analysis of FGFR4 on different lysates with Rabbit anti-FGFR4 antibody (HA721578) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Huh7 cell lysate
Lane 3: SW480 cell lysate
Lane 4: Raji cell lysate
Lane 5: PANC-1 cell lysate
Lane 6: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88/110 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721578) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of Caco-2 cells labeling FGFR4.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721578, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"