GluA4 Recombinant Rabbit Monoclonal Antibody [JE36-81]
Catalog# HA721582
GluA4 Recombinant Rabbit Monoclonal Antibody [JE36-81]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
GluA4 Recombinant Rabbit Monoclonal Antibody [JE36-81]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human GluA4 aa 853-902 / 902.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 101 kDa
Positive Control
Mouse cerebellum tissue lysate, rat cerebellum tissue lysate, SH-SY5Y.
Conjugation
unconjugated
Clone Number
JE36-81
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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FC
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1:500-1:1,000
Target
Function
Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
Background References
1. Kato A.S., Gill M.B., Ho M.T., Yu H., Tu Y., Siuda E.R., Wang H., Qian Y.W., Nisenbaum E.S., Bredt D.S. Hippocampal AMPA receptor gating controlled by both TARP and cornichon proteins. Neuron 68:1082-1096 (2010).
Subcellular Location
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Synonyms
AMPA 4 antibody
AMPA selective glutamate receptor 4 antibody
AMPA-selective glutamate receptor 4 antibody
AMPA4 antibody
GluA 4 antibody
GluA4 antibody
GluR 4 antibody
GluR D antibody
GluR-4 antibody
GluR-D antibody
ExpandImages
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Western blot analysis of GluA4 on different lysates with Rabbit anti-GluA4 antibody (HA721582) at 1/1,000 dilution.
Lane 1: Mouse cerebellum tissue lysate (RIPA lysis)
Lane 2: Mouse cerebellum tissue lysate (hot lysis)
Lane 3: Rat cerebellum tissue lysate (RIPA lysis)
Lane 4: Rat cerebellum tissue lysate (hot lysis)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 101 kDa
Observed band size: 110/60 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721582) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of SH-SY5Y cells labeling GluA4.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721582, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"