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PF4 Recombinant Rabbit Monoclonal Antibody [JE31-48]

Usd: 385

Specification

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Catalog# HA721584

PF4 Recombinant Rabbit Monoclonal Antibody [JE31-48]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PF4 Recombinant Rabbit Monoclonal Antibody [JE31-48]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PF4 aa 52-101 / 101.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 11 kDa

Positive Control

Human plasma lysates, Mouse spleen tissue lysate, Mouse platelets lysate, Rat spleen tissue lysate, human liver tissue, human bone marrow tissue, rat bone marrow tissue.

Conjugation

unconjugated

Clone Number

JE31-48

RRID

AB_3072697

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. It is usually found in a complex with proteoglycan. The gene for human PF4 is located on human chromosome 4. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3-B.

Background References

1. Liu Q et al. Anti-PF4 antibodies associated with disease severity in COVID-19. Proc Natl Acad Sci U S A. 2022 Nov

2. Vayne C et al. PF4 Immunoassays in Vaccine-Induced Thrombotic Thrombocytopenia. N Engl J Med. 2021 Jul

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02776 Human

SwissProt: Q9Z126 Mouse

SwissProt: P06765 Rat

Synonyms

C-X-C motif chemokine 4 antibody

Chemokine (C X C motif) ligand 4 antibody

Chemokine (CXC motif) ligand 4 antibody

chemokine ligand 4 antibody

CXCL 4 antibody

CXCL4 antibody

Iroplact antibody

MGC138298 antibody

Oncostatin A antibody

Oncostatin-A antibody

Expand

Images

  • Western blot analysis of PF4 on human plasma lysates with Rabbit anti-PF4 antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 11 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PF4 on human plasma lysates with Rabbit anti-PF4 antibody (HA721584) at 1/1,000 dilution.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 11 kDa
    Observed band size: 14 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PF4 on different lysates with Rabbit anti-PF4 antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate (30 µg/Lane)<br />Lane 2: Mouse platelets lysate (6 µg/Lane)<br />Lane 3: Rat spleen tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 11 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PF4 on different lysates with Rabbit anti-PF4 antibody (HA721584) at 1/1,000 dilution.

    Lane 1: Mouse spleen tissue lysate (30 µg/Lane)
    Lane 2: Mouse platelets lysate (6 µg/Lane)
    Lane 3: Rat spleen tissue lysate (30 µg/Lane)

    Predicted band size: 11 kDa
    Observed band size: 14 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PF4 antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PF4 antibody (HA721584) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721584) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-PF4 antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-PF4 antibody (HA721584) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721584) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat bone marrow tissue with Rabbit anti-PF4 antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721584" style="font-weight: bold;text-decoration: underline;">HA721584</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat bone marrow tissue with Rabbit anti-PF4 antibody (HA721584) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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