CoREST Recombinant Rabbit Monoclonal Antibody [JE35-15]
Catalog# HA721585
CoREST Recombinant Rabbit Monoclonal Antibody [JE35-15]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CoREST Recombinant Rabbit Monoclonal Antibody [JE35-15]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CoREST aa 1-50 / 485.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 65 kDa
Positive Control
K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, U-2 OS cell lysate, HT-29 cell lysate, human appendix tissue, human testis tissue, mouse brain tissue, rat colon tissue.
Conjugation
unconjugated
Clone Number
JE35-15
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:200-1:1,000
Target
Function
Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.
Background References
1. Maksour S et al. More than a Corepressor: The Role of CoREST Proteins in Neurodevelopment. eNeuro. 2020 Mar
2. Garcia-Martinez L et al. Endocrine resistance and breast cancer plasticity are controlled by CoREST. Nat Struct Mol Biol. 2022 Nov
Subcellular Location
Nucleus.
Synonyms
COREST antibody
KIAA0071 antibody
Nr2b2 antibody
Nuclear receptor coregulator 1 antibody
Nuclear receptor subfamily 2 group B member 2 antibody
Protein CoREST antibody
RCOR antibody
Rcor1 antibody
RCOR1_HUMAN antibody
REST corepressor 1 antibody
ExpandImages
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Western blot analysis of CoREST on different lysates with Rabbit anti-CoREST antibody (HA721585) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: U-2 OS cell lysate
Lane 8: HT-29 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65/26 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721585) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CoREST antibody (HA721585) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CoREST antibody (HA721585) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CoREST antibody (HA721585) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CoREST antibody (HA721585) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"