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CoREST Recombinant Rabbit Monoclonal Antibody [JE35-15]

Usd: 385

Specification

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Catalog# HA721585

CoREST Recombinant Rabbit Monoclonal Antibody [JE35-15]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CoREST Recombinant Rabbit Monoclonal Antibody [JE35-15]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CoREST aa 1-50 / 485.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 65 kDa

Positive Control

K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, U-2 OS cell lysate, HT-29 cell lysate, human appendix tissue, human testis tissue, mouse brain tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

JE35-15

RRID

AB_3072698

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.

Background References

1. Maksour S et al. More than a Corepressor: The Role of CoREST Proteins in Neurodevelopment. eNeuro. 2020 Mar

2. Garcia-Martinez L et al. Endocrine resistance and breast cancer plasticity are controlled by CoREST. Nat Struct Mol Biol. 2022 Nov

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9UKL0 Human

SwissProt: Q8CFE3 Mouse

SwissProt: P49743 Rat

Synonyms

COREST antibody

KIAA0071 antibody

Nr2b2 antibody

Nuclear receptor coregulator 1 antibody

Nuclear receptor subfamily 2 group B member 2 antibody

Protein CoREST antibody

RCOR antibody

Rcor1 antibody

RCOR1_HUMAN antibody

REST corepressor 1 antibody

Expand

Images

  • Western blot analysis of CoREST on different lysates with Rabbit anti-CoREST antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: HepG2 cell lysate<br />Lane 6: A549 cell lysate<br />Lane 7: U-2 OS cell lysate<br />Lane 8: HT-29 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 65/26 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CoREST on different lysates with Rabbit anti-CoREST antibody (HA721585) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: A549 cell lysate
    Lane 7: U-2 OS cell lysate
    Lane 8: HT-29 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 65/26 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721585) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CoREST antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CoREST antibody (HA721585) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CoREST antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CoREST antibody (HA721585) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CoREST antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CoREST antibody (HA721585) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CoREST antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721585" style="font-weight: bold;text-decoration: underline;">HA721585</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CoREST antibody (HA721585) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721585) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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