IFT88 Recombinant Rabbit Monoclonal Antibody [JE64-77]
Catalog# HA721590
IFT88 Recombinant Rabbit Monoclonal Antibody [JE64-77]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
IFT88 Recombinant Rabbit Monoclonal Antibody [JE64-77]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human IFT88 aa 1-50 / 824.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 93 kDa
Positive Control
HEK-293 cell lysate, 293T cell lysate, mouse testis tissue lysate, rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-77
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Intraflagellar transport protein 88 homolog is a protein that is encoded by the IFT88 gene. This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. In 2012 a mutation was found to be responsible for a novel form of ciliopathy and anosmia in humans capable of remedy in mice by adenoviral mediated gene therapy. IFT88 has been shown to interact with BAT2 and WDR62.[8][9] WDR62 is required for IFT88 localization to the cilia basal body and the cilia axoneme.
Background References
1. Kitamura A et al. Ift88 is involved in mandibular development. J Anat. 2020 Feb
2. Wang S et al. IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy. Am J Physiol Renal Physiol. 2021 Sep
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cell projection, cilium, cilium basal body, flagellum.
Synonyms
D13S1056E antibody
DAF19 antibody
hTg737 antibody
Ift88 antibody
IFT88_HUMAN antibody
Intraflagellar transport 88 homolog antibody
Intraflagellar transport protein 88 homolog antibody
MGC26259 antibody
Polaris homolog antibody
Probe hTg737 (polycystic kidney disease, autosomal recessive) antibody
ExpandImages
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Western blot analysis of IFT88 on different lysates with Rabbit anti-IFT88 antibody (HA721590) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (40 µg/Lane)
Lane 4: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721590) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"