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KIF4A Recombinant Rabbit Monoclonal Antibody [JE38-91]

Usd: 385

Specification

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Catalog# HA721591

KIF4A Recombinant Rabbit Monoclonal Antibody [JE38-91]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

KIF4A Recombinant Rabbit Monoclonal Antibody [JE38-91]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human KIF4A aa 31-80 / 1,232.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 140 kDa

Positive Control

HeLa cell lysate, U-87 MG cell lysate, 293T cell lysate, SW480 cell lysate, mouse thymus tissue lysate, NIH/3T3 cell lysate, human lung carcinoma tissue.

Conjugation

unconjugated

Clone Number

JE38-91

RRID

AB_3072704

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:1,000

Target

Function

Kinesin family member 4A is a protein that in humans is encoded by the KIF4A gene. Kinesins, such as KIF4A, are microtubule-based motor proteins that generate directional movement along microtubules. They are involved in many crucial cellular processes, including cell division. KIF4A has been shown to interact with HMG20B and DNMT3B. NTCP is the entry receptor for both Hepatitis B (HBV) and Hepatitis D viruses (HDV). KIF4 was found to play an essential role in HBV and HDV infection through its regulation of the retrograde transport of NTCP from the cytoplasm to the cell surface where it acts as a receptor for HBV/HDV infection.

Background References

1. Sun X et al. KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma. Thorac Cancer. 2021 Feb

2. Chen J et al. KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion. Biomed Res Int. 2021 Nov

Subcellular Location

Nucleus matrix, Cytoplasm, cytoskeleton, spindle, Midbody, Chromosome.

UNIPROT

SwissProt: O95239 Human

SwissProt: P33174 Mouse

Synonyms

Chromokinesin antibody

Chromokinesin-A antibody

Chromosome associated kinesin KIF4A antibody

Chromosome-associated kinesin KIF4A antibody

FLJ12530 antibody

FLJ12655 antibody

FLJ14204 antibody

FLJ20631 antibody

HSA271784 antibody

KIF4 antibody

Expand

Images

  • Western blot analysis of KIF4A on different lysates with Rabbit anti-KIF4A antibody (<a href="/products/HA721591" style="font-weight: bold;text-decoration: underline;">HA721591</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane)<br />Lane 4: SW480 cell lysate (20 µg/Lane)<br />Lane 5: Mouse thymus tissue lysate (40 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 140 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 6 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721591" style="font-weight: bold;text-decoration: underline;">HA721591</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KIF4A on different lysates with Rabbit anti-KIF4A antibody (HA721591) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: SW480 cell lysate (20 µg/Lane)
    Lane 5: Mouse thymus tissue lysate (40 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)

    Predicted band size: 140 kDa
    Observed band size: 160 kDa

    Exposure time: 6 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721591) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of KIF4A on different lysates with Rabbit anti-KIF4A antibody (<a href="/products/HA721591" style="font-weight: bold;text-decoration: underline;">HA721591</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-KIF4A KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 140 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 60 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721591" style="font-weight: bold;text-decoration: underline;">HA721591</a>) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of KIF4A on different lysates with Rabbit anti-KIF4A antibody (HA721591) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-KIF4A KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 140 kDa
    Observed band size: 160 kDa

    Exposure time: 60 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721591) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-KIF4A antibody (<a href="/products/HA721591" style="font-weight: bold;text-decoration: underline;">HA721591</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721591" style="font-weight: bold;text-decoration: underline;">HA721591</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-KIF4A antibody (HA721591) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721591) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"