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POLD1 Recombinant Rabbit Monoclonal Antibody [JE35-73]

Usd: 385

Specification

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Catalog# HA721597

POLD1 Recombinant Rabbit Monoclonal Antibody [JE35-73]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

POLD1 Recombinant Rabbit Monoclonal Antibody [JE35-73]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human POLD1 aa 1,001-1,050 / 1,107.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 124 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, 293T cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, PC-3M cell lysate, mouse testis tissue lysate, rat testis tissue lysate, SW480 cell lysate.

Conjugation

unconjugated

Clone Number

JE35-73

RRID

AB_3072710

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

The gene polymerase delta 1 (POLD1) encodes the large, POLD1/p125, catalytic subunit of the DNA polymerase delta (Polδ) complex. The Polδ enzyme is responsible for synthesizing the lagging strand of DNA, and has also been implicated in some activities at the leading strand. The POLD1/p125 subunit encodes both DNA polymerizing and exonuclease domains, which provide the protein an important second function in proofreading to ensure replication accuracy during DNA synthesis, and in a number of types of replication-linked DNA repair following DNA damage. Germline mutations impairing activity of POLD1 have been implicated in several types of hereditary cancer, in some sporadic cancers, and in a developmental syndrome of premature aging, Mandibular hypoplasia, Deafness, and Progeroid features and Lipodystrophy (MDPL/MDP syndrome). Studies of POLD1 emphasize the importance of maintaining genomic stability to limit tumorigenesis. It is currently unclear whether the enhanced tumorigenesis associated with POLD1 defects is the result of increased base substitutions or due to fork collapse and production of DNA double strand breaks (DSBs). Recent reviews have addressed important functions of POLD1 and Polδ.

Background References

1. Ma X et al. POLE/POLD1 mutation and tumor immunotherapy. J Exp Clin Cancer Res. 2022 Jul

2. Magrin L et al. POLE, POLD1, and NTHL1: the last but not the least hereditary cancer-predisposing genes. Oncogene. 2021 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P28340 Human

SwissProt: P52431 Mouse

SwissProt: O54747 Rat

Synonyms

Polymerase (DNA directed) delta 1 catalytic subunit antibody

CDC2 antibody

CDC2 homolog antibody

CRCS10 antibody

DNA directed DNA polymerase delta 1 antibody

DNA directed polymerase delta 1 antibody

DNA pol delta 1 antibody

DNA polymerase delta catalytic subunit antibody

DNA polymerase subunit delta p125 antibody

DPOD1_HUMAN antibody

Expand

Images

  • Western blot analysis of POLD1 on different lysates with Rabbit anti-POLD1 antibody (<a href="/products/HA721597" style="font-weight: bold;text-decoration: underline;">HA721597</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 6: PC-3M cell lysate (20 µg/Lane)<br />Lane 7: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 8: Rat testis tissue lysate (40 µg/Lane)<br />Lane 9: SW480 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 124 kDa<br />Observed band size: 124 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721597" style="font-weight: bold;text-decoration: underline;">HA721597</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of POLD1 on different lysates with Rabbit anti-POLD1 antibody (HA721597) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: RAW264.7 cell lysate (20 µg/Lane)
    Lane 6: PC-3M cell lysate (20 µg/Lane)
    Lane 7: Mouse testis tissue lysate (40 µg/Lane)
    Lane 8: Rat testis tissue lysate (40 µg/Lane)
    Lane 9: SW480 cell lysate (20 µg/Lane)

    Predicted band size: 124 kDa
    Observed band size: 124 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721597) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded <br />rat testis tissue with Rabbit anti-POLD1 antibody (<a href="/products/HA721597" style="font-weight: bold;text-decoration: underline;">HA721597</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721597" style="font-weight: bold;text-decoration: underline;">HA721597</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded
    rat testis tissue with Rabbit anti-POLD1 antibody (HA721597) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721597) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation