Mouse IL-6 Recombinant Rabbit Monoclonal Antibody [PS01-74] - BSA and Azide free (Capture/Detector)
Overview
Product Name
Mouse IL-6 Recombinant Rabbit Monoclonal Antibody [PS01-74] - BSA and Azide free (Capture/Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL-6 aa 25-211.
Species Reactivity
Mouse
Validated Applications
ELISA(Cap), ELISA(Det)
Conjugation
unconjugated
Clone Number
PS01-74
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
1:1,000
Target
Function
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
Background References
1. Ibrahim ML. et. al. Expression profiles and function of IL6 in polymorphonuclear myeloid-derived suppressor cells. Cancer Immunol Immunother. 2020 Nov
2. Galoian K. et. al. Analysis of IL6-protein complexes in chondrosarcoma. Biomed Rep. 2018 Jan
Subcellular Location
Secreted.
UNIPROT
Synonyms
Interleukin BSF 2 antibody
B cell differentiation factor antibody
B cell stimulatory factor 2 antibody
B-cell stimulatory factor 2 antibody
BSF 2 antibody
BSF-2 antibody
BSF2 antibody
CDF antibody
CTL differentiation factor antibody
Cytotoxic T cell differentiation factor antibody
ExpandImages
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Sandwich ELISA analysis of Mouse IL-6 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721600) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-6 protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721601, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"