Mouse IL-9 Recombinant Rabbit Monoclonal Antibody [PS01-81] - BSA and Azide free (Capture)
Overview
Product Name
Mouse IL-9 Recombinant Rabbit Monoclonal Antibody [PS01-81] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IL-9 aa 19-144 (HA210631).
Species Reactivity
Mouse
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Mouse IL-9 protein (HA210631).
Conjugation
unconjugated
Clone Number
PS01-81
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-82] to Mouse IL-9 antibody (Detector) (HA721604) and Recombinant Mouse IL-9 protein (HA210631) as the standard. The reference range value is 7.81-1,000 pg/mL.
Target
Function
Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites. Affects intestinal epithelial permeability and adaptive immunity. In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells. Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG. Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs. Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5.
Background References
1. Townsend J.M., Fallon G.P., Matthews J.D., Smith P., Jolin E.H., McKenzie N.A. IL-9-deficient mice establish fundamental roles for IL-9 in pulmonary mastocytosis and goblet cell hyperplasia but not T cell development. Immunity 13:573-583 (2000).
2. Demoulin J.B., Van Roost E., Stevens M., Groner B., Renauld J.C. Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9. J. Biol. Chem. 274:25855-25861 (1999).
Subcellular Location
Secreted.
Synonyms
Cytokine P40 antibody
Homolog of mouse T cell and mast cell growth factor 40 antibody
HP40 antibody
IL 9 antibody
IL-9 antibody
Il9 antibody
IL9_HUMAN antibody
Interleukin 9 antibody
Interleukin-9 antibody
Mast cell growth factor antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse IL-9 matched pair antibodies<br /><br />Capture: <a href="/products/HA721603" style="font-weight: bold;text-decoration: underline;">HA721603</a>, Mouse IL-9 Rabbit mAb [PS01-81]<br />Detector: <a href="/products/HA721604" style="font-weight: bold;text-decoration: underline;">HA721604</a>, Mouse IL-9 Rabbit mAb [PS01-82]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721603" style="font-weight: bold;text-decoration: underline;">HA721603</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-9 protein (<a href="/products/HA210631" style="font-weight: bold;text-decoration: underline;">HA210631</a>) starting from 800 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA721604" style="font-weight: bold;text-decoration: underline;">HA721604</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721603_1.jpg?v=20260429161645)
Sandwich ELISA analysis of Mouse IL-9 matched pair antibodies
Capture: HA721603, Mouse IL-9 Rabbit mAb [PS01-81]
Detector: HA721604, Mouse IL-9 Rabbit mAb [PS01-82]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721603) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-9 protein (HA210631) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721604, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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