Biotin Conjugated Mouse IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-92] - Detector
Overview
Product Name
Biotin Conjugated Mouse IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-92] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse IL17A aa 26-158 (HA210791).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse IL-17A protein (HA210791).
Conjugation
Biotin
Clone Number
PS01-92
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-91] to Mouse IL-17A antibody (Capture) (HA721606) and Recombinant Mouse IL-17A protein (HA210791) as the standard. The reference range value is 7.81-1,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance.
Background References
1. Liang S.C., Long A.J., Bennett F., Whitters M.J., Karim R., Collins M., Goldman S.J., Dunussi-Joannopoulos K., Williams C.M., Wright J.F., Fouser L.A. An IL-17F/A heterodimer protein is produced by mouse Th17 cells and induces airway neutrophil recruitment. J. Immunol. 179:7791-7799 (2007).
2. Kuestner R.E., Taft D.W., Haran A., Brandt C.S., Brender T., Lum K., Harder B., Okada S., Ostrander C.D., Kreindler J.L., Aujla S.J. Identification of the IL-17 receptor related molecule IL-17RC as the receptor for IL-17F. J. Immunol. 179:5462-5473 (2007).
Subcellular Location
Secreted.
Synonyms
CTLA 8 antibody
CTLA-8 antibody
CTLA8 antibody
cytotoxic T lymphocyte associated antigen 8 antibody
Cytotoxic T lymphocyte associated protein 8 antibody
Cytotoxic T lymphocyte associated serine esterase 8 antibody
Cytotoxic T-lymphocyte-associated antigen 8 antibody
IL 17 antibody
IL 17A antibody
IL-17 antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies<br /><br />Capture: <a href="/products/HA721606" style="font-weight: bold;text-decoration: underline;">HA721606</a>, Mouse IL-17A Rabbit mAb [PS01-91]<br />Detector: <a href="/products/HA721607" style="font-weight: bold;text-decoration: underline;">HA721607</a>, Mouse IL-17A Rabbit mAb [PS01-92]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721606" style="font-weight: bold;text-decoration: underline;">HA721606</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17A protein (<a href="/products/HA210791" style="font-weight: bold;text-decoration: underline;">HA210791</a>) protein starting from 800 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA721607" style="font-weight: bold;text-decoration: underline;">HA721607</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721608_1.jpg?v=20260604175046)
Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies
Capture: HA721606, Mouse IL-17A Rabbit mAb [PS01-91]
Detector: HA721607, Mouse IL-17A Rabbit mAb [PS01-92]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721606) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17A protein (HA210791) protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721607, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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