Skip to content

Rat IL-6 Recombinant Rabbit Monoclonal Antibody [PS01-90] - BSA and Azide free (Capture)

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA721615

Rat IL-6 Recombinant Rabbit Monoclonal Antibody [PS01-90] - BSA and Azide free (Capture)

Application

  • ELISA(Cap)

Reactivity

  • Rat

Pair: Capture
Pair: Detector
Pair: Protein
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Rat IL-6 Recombinant Rabbit Monoclonal Antibody [PS01-90] - BSA and Azide free (Capture)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant full length protein rat IL-6.

Species Reactivity

Rat

Validated Applications

ELISA(Cap)

Conjugation

unconjugated

Clone Number

PS01-90

RRID

AB_3072728

Product Features

Form

Liquid

Concentration

1 mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Cap)

  • 1:1,000

Target

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

Background References

1. Timper K., Denson J.L., Steculorum S.M., Heilinger C., Engstroem-Ruud L., Wunderlich C.M., Rose-John S., Wunderlich F.T., Bruening J.C. IL-6 Improves Energy and Glucose Homeostasis in Obesity via Enhanced Central IL-6 trans-Signaling. Cell Rep. 19:267-280 (2017)

2. Ivanov I.I., McKenzie B.S., Zhou L., Tadokoro C.E., Lepelley A., Lafaille J.J., Cua D.J., Littman D.R. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 126:1121-1133 (2006)

Subcellular Location

Secreted.

UNIPROT

SwissProt: P20607 Rat

Entrez Gene: 24498 Rat

Unigene: 9873 Rat

Synonyms

Interleukin BSF 2 antibody

B cell differentiation factor antibody

B cell stimulatory factor 2 antibody

B-cell stimulatory factor 2 antibody

BSF 2 antibody

BSF-2 antibody

BSF2 antibody

CDF antibody

CTL differentiation factor antibody

Cytotoxic T cell differentiation factor antibody

Expand

Images

  • Sandwich ELISA analysis of Rat IL-6 matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721615" style="font-weight: bold;text-decoration: underline;">HA721615</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-6 protein starting from 30,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA721616" style="font-weight: bold;text-decoration: underline;">HA721616</a>) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of Rat IL-6 matched pair antibodies

    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721615) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-6 protein starting from 30,000 pg/ml to 0 pg/ml and detect antibody (HA721616) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"