Biotin Conjugated Rat IFN gamma Recombinant Rabbit Monoclonal Antibody [PS01-97] - Detector
Overview
Product Name
Biotin Conjugated Rat IFN gamma Recombinant Rabbit Monoclonal Antibody [PS01-97] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Rat IFN gamma aa 24-156 (HA210577).
Species Reactivity
Rat
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant standard Rat IFN gamma protein (HA210577).
Conjugation
Biotin
Clone Number
PS01-97
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-96] to Rat IFN gamma (Capture) (HA721618) and Recombinant Rat IFN gamma protein (HA210577) as the standard. The reference range value is 156.25-10,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Background References
1. Greenlund A.C., Schreiber R.D., Goeddel D.V., Pennica D. Interferon-gamma induces receptor dimerization in solution and on cells. J. Biol. Chem. 268:18103-18110 (1993).
2. Hisamatsu H., Shimbara N., Saito Y., Kristensen P., Hendil K.B., Fujiwara T., Takahashi E., Tanahashi N., Tamura T., Ichihara A., Tanaka K. Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma. J. Exp. Med. 183:1807-1816 (1996).
Subcellular Location
Secreted
UNIPROT
Synonyms
IF 1 antibody
IFG antibody
IFI antibody
ifn g antibody
IFN gamma antibody
IFN immune antibody
IFN, immune antibody
IFN-gamma antibody
IFNG antibody
IFNG_HUMAN antibody
ExpandImages
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![Sandwich ELISA analysis of Rat IFN gamma protein matched pair antibodies<br /><br />Capture: <a href="/products/HA721618" style="font-weight: bold;text-decoration: underline;">HA721618</a>, Rat IFN gamma Rabbit mAb [PS01-96]<br />Detector: <a href="/products/HA721619" style="font-weight: bold;text-decoration: underline;">HA721619</a>, Rat IFN gamma Rabbit mAb [PS01-97]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721618" style="font-weight: bold;text-decoration: underline;">HA721618</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IFN gamma protein (<a href="/products/HA210577" style="font-weight: bold;text-decoration: underline;">HA210577</a>) starting from 10,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA721619" style="font-weight: bold;text-decoration: underline;">HA721619</a>, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721620_1.jpg?v=20260917230314)
Sandwich ELISA analysis of Rat IFN gamma protein matched pair antibodies
Capture: HA721618, Rat IFN gamma Rabbit mAb [PS01-96]
Detector: HA721619, Rat IFN gamma Rabbit mAb [PS01-97]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721618) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IFN gamma protein (HA210577) starting from 10,000 pg/mL to 0 pg/mL and detect antibody (HA721619, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"