HCLS1 Recombinant Rabbit Monoclonal Antibody [PSH01-12]
Catalog# HA721624
HCLS1 Recombinant Rabbit Monoclonal Antibody [PSH01-12]
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WB
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IHC-P
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IF-Cell
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FC
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IF-Tissue
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Human
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HA750688
不含抗保成分
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unconjugated
Overview
Product Name
HCLS1 Recombinant Rabbit Monoclonal Antibody [PSH01-12]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human HCLS1 aa 201-450 / 486
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell, FC, IF-Tissue
Molecular Weight
Predicted band size: 54 kDa
Positive Control
Ramos cell lysate, Daudi cell lysate, Raji cell lysate, Jurkat cell lysate, HL-60 cell lysate, human lung carcinoma tissue, human liver tissue, human brain tissue, HL-60, Jurkat.
Conjugation
unconjugated
Clone Number
PSH01-12
RRID
Reactivity Data
| WB | IHC-P | IF-Cell | FC | IF-Tissue | |
|---|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200-1:5,000
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IF-Cell
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1:100-1:250
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FC
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1:500-1:1,000
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IF-Tissue
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1:1,000
Target
Function
Hematopoietic lineage cell-specific protein is a protein that in humans is encoded by the HCLS1 gene. Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and protein kinase binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of protein phosphorylation; and regulation of transcription, DNA-templated. Located in cytosol; nucleus; and plasma membrane. Part of transcription regulator complex. HCLS1 has been shown to interact with Caspase 3.
Background References
1. Quatrana A et al. Hsa-miR223-3p circulating level is upregulated in Friedreich\'s ataxia and inversely associated with HCLS1 associated protein X-1, HAX-1. Hum Mol Genet. 2022 Jun
Subcellular Location
Membrane, Cytoplasm, Mitochondrion.
UNIPROT
Synonyms
Cortactin like antibody
CTTNL antibody
HCLS 1 antibody
Hcls1 antibody
HCLS1_HUMAN antibody
Hematopoietic cell specific Lyn substrate 1 antibody
Hematopoietic cell-specific LYN substrate 1 antibody
Hematopoietic lineage cell-specific protein antibody
HS 1 antibody
HS1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of HCLS1 on different lysates with Rabbit anti-HCLS1 antibody (HA721624) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: Ramos cell lysate (20 µg/Lane)
Lane 2: Daudi cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: HEK-293 cell lysate (negative) (20 µg/Lane)
Lane 6: HL-60 cell lysate (20 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 75 kDa
Exposure time: 42 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721624) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-HCLS1 antibody (HA721624) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721624) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HCLS1 antibody (HA721624) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721624) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-HCLS1 antibody (HA721624) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721624) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunocytochemistry analysis of HL-60 (positive) and HEK-293 (negative) labeling HCLS1 with Rabbit anti-HCLS1 antibody (HA721624) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HCLS1 antibody (HA721624) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
Immunocytochemistry analysis of Jurkat (positive) and HEK-293 (negative) labeling HCLS1 with Rabbit anti-HCLS1 antibody (HA721624) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HCLS1 antibody (HA721624) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Application: IF-Tissue
Species: Human
Site: lung carcinoma
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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