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Phospho-mTOR (S2481) Recombinant Rabbit Monoclonal Antibody [JE59-62]

Usd: 385

Specification

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Catalog# HA721632

Phospho-mTOR (S2481) Recombinant Rabbit Monoclonal Antibody [JE59-62]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-mTOR (S2481) Recombinant Rabbit Monoclonal Antibody [JE59-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human mTOR.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 289 kDa

Positive Control

HeLa whole cell lysate, HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate.

Conjugation

unconjugated

Clone Number

JE59-62

RRID

AB_3072745

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

The mammalian target of rapamycin (mTOR), also referred to as the mechanistic target of rapamycin, and sometimes called FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. mTOR is a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases. mTOR links with other proteins and serves as a core component of two distinct protein complexes, mTOR complex 1 and mTOR complex 2, which regulate different cellular processes. In particular, as a core component of both complexes, mTOR functions as a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, autophagy, and transcription. As a core component of mTORC2, mTOR also functions as a tyrosine protein kinase that promotes the activation of insulin receptors and insulin-like growth factor 1 receptors. mTORC2 has also been implicated in the control and maintenance of the actin cytoskeleton.

Background References

1. Deleyto-Seldas N et al. The mTOR-Autophagy Axis and the Control of Metabolism. Front Cell Dev Biol. 2021 Jul

2. Chen Y et al. Research progress of mTOR inhibitors. Eur J Med Chem. 2020 Dec

Subcellular Location

Endoplasmic reticulum membrane, Golgi apparatus membrane, Mitochondrion outer membrane, Lysosome, Cytoplasm, Nucleus, Microsome membrane, Lysosome membrane, Cytoplasmic vesicle, phagosome.

UNIPROT

SwissProt: P42345 Human

Synonyms

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody

FK506 binding protein 12 rapamycin associated protein 1 antibody

FK506 binding protein 12 rapamycin associated protein 2 antibody

FK506 binding protein 12 rapamycin complex associated protein 1 antibody

FK506-binding protein 12-rapamycin complex-associated protein 1 antibody

FKBP rapamycin associated protein antibody

FKBP12 rapamycin complex associated protein antibody

FKBP12-rapamycin complex-associated protein 1 antibody

FKBP12-rapamycin complex-associated protein antibody

FLJ44809 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-mTOR (S2481) on different lysates with Rabbit anti-Phospho-mTOR (S2481) antibody (<a href="/products/HA721632" style="font-weight: bold;text-decoration: underline;">HA721632</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa whole cell lysate<br />Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 289 kDa<br />Observed band size: 289 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721632" style="font-weight: bold;text-decoration: underline;">HA721632</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-mTOR (S2481) on different lysates with Rabbit anti-Phospho-mTOR (S2481) antibody (HA721632) at 1/5,000 dilution.

    Lane 1: HeLa whole cell lysate
    Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 289 kDa
    Observed band size: 289 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721632) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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