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POLDIP2 Recombinant Rabbit Monoclonal Antibody [PSH01-20]

Usd: 385

Specification

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Catalog# HA721637

POLDIP2 Recombinant Rabbit Monoclonal Antibody [PSH01-20]

Application

  • WB

Reactivity

  • Human

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

POLDIP2 Recombinant Rabbit Monoclonal Antibody [PSH01-20]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human POLDIP2 aa 31-368 / 368.

Species Reactivity

Human, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

HepG2 cell lysate, HL-60 cell lysate, U-87 MG cell lysate, rat spleen tissue lysate, rat thymus tissue lysate, rat kidney tissue lysate, rat lung tissue lysate.

Conjugation

unconjugated

Clone Number

PSH01-20

RRID

AB_3072750

Reactivity Data

WB
Human
Tested Tested
Rat
Tested Tested
Mouse
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Polymerase delta-interacting protein 2 also known as Polymerase delta-interacting protein of 38 kDa (PDIP38) is encoded by the POLDIP2 gene in humans. This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. The encoded protein also interacts with proliferating cell nuclear antigen. Some transcripts of this gene overlap in a tail-to-tail orientation with the gene for tumor necrosis factor, alpha-induced protein 1 (TNFAIP1). POLDIP2 / PDIP38 is a moonlighting protein that has been identified in both the nucleus and the mitochondrion.

Background References

1. Liu K et al. Capsaicin ameliorates diabetic retinopathy by inhibiting poldip2-induced oxidative stress. Redox Biol. 2022 Oct

2. Dolmatova EV et al. Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation. Cardiovasc Res. 2022 Aug

Subcellular Location

Mitochondrion matrix, Nucleus.

UNIPROT

SwissProt: Q9Y2S7 Human

Entrez Gene: 287544 Rat

Synonyms

38 kDa DNA polymerase delta interaction protein antibody

delta interacting protein 2 antibody

DNA polymerase delta interaction protein p38 antibody

p38 antibody

PDIP 2 antibody

PDIP 38 antibody

PDIP2 antibody

PDIP2_HUMAN antibody

PDIP38 antibody

POLD 4 antibody

Expand

Images

  • Western blot analysis of POLDIP2 on different lysates with Rabbit anti-POLDIP2 antibody (<a href="/products/HA721637" style="font-weight: bold;text-decoration: underline;">HA721637</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HL-60 cell lysate (20 µg/Lane)<br />Lane 3: U-87 MG cell lysate (20 µg/Lane)<br />Lane 4: Rat spleen tissue lysate (40 µg/Lane)<br />Lane 5: Rat thymus tissue lysate (40 µg/Lane)<br />Lane 6: Rat kidney tissue lysate (40 µg/Lane)<br />Lane 7: Rat lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 8 minutes 54 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721637" style="font-weight: bold;text-decoration: underline;">HA721637</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of POLDIP2 on different lysates with Rabbit anti-POLDIP2 antibody (HA721637) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HL-60 cell lysate (20 µg/Lane)
    Lane 3: U-87 MG cell lysate (20 µg/Lane)
    Lane 4: Rat spleen tissue lysate (40 µg/Lane)
    Lane 5: Rat thymus tissue lysate (40 µg/Lane)
    Lane 6: Rat kidney tissue lysate (40 µg/Lane)
    Lane 7: Rat lung tissue lysate (40 µg/Lane)

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 8 minutes 54 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721637) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"