POLDIP2 Recombinant Rabbit Monoclonal Antibody [PSH01-20]
Catalog# HA721637
POLDIP2 Recombinant Rabbit Monoclonal Antibody [PSH01-20]
-
WB
-
Human
-
Rat
-
unconjugated
Overview
Product Name
POLDIP2 Recombinant Rabbit Monoclonal Antibody [PSH01-20]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human POLDIP2 aa 31-368 / 368.
Species Reactivity
Human, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
HepG2 cell lysate, HL-60 cell lysate, U-87 MG cell lysate, rat spleen tissue lysate, rat thymus tissue lysate, rat kidney tissue lysate, rat lung tissue lysate.
Conjugation
unconjugated
Clone Number
PSH01-20
RRID
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Rat |
|
| Mouse |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Polymerase delta-interacting protein 2 also known as Polymerase delta-interacting protein of 38 kDa (PDIP38) is encoded by the POLDIP2 gene in humans. This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. The encoded protein also interacts with proliferating cell nuclear antigen. Some transcripts of this gene overlap in a tail-to-tail orientation with the gene for tumor necrosis factor, alpha-induced protein 1 (TNFAIP1). POLDIP2 / PDIP38 is a moonlighting protein that has been identified in both the nucleus and the mitochondrion.
Background References
1. Liu K et al. Capsaicin ameliorates diabetic retinopathy by inhibiting poldip2-induced oxidative stress. Redox Biol. 2022 Oct
2. Dolmatova EV et al. Endothelial Poldip2 regulates sepsis-induced lung injury via Rho pathway activation. Cardiovasc Res. 2022 Aug
Subcellular Location
Mitochondrion matrix, Nucleus.
Synonyms
38 kDa DNA polymerase delta interaction protein antibody
delta interacting protein 2 antibody
DNA polymerase delta interaction protein p38 antibody
p38 antibody
PDIP 2 antibody
PDIP 38 antibody
PDIP2 antibody
PDIP2_HUMAN antibody
PDIP38 antibody
POLD 4 antibody
ExpandImages
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Western blot analysis of POLDIP2 on different lysates with Rabbit anti-POLDIP2 antibody (HA721637) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HL-60 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Lane 5: Rat thymus tissue lysate (40 µg/Lane)
Lane 6: Rat kidney tissue lysate (40 µg/Lane)
Lane 7: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 8 minutes 54 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721637) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"