Apo-H Recombinant Rabbit Monoclonal Antibody [JE37-02]
Catalog# HA721649
Apo-H Recombinant Rabbit Monoclonal Antibody [JE37-02]
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WB
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Human
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unconjugated
Overview
Product Name
Apo-H Recombinant Rabbit Monoclonal Antibody [JE37-02]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Apo-H aa 71-120 / 345.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 38 kDa
Positive Control
Human liver tissue lysates.
Conjugation
unconjugated
Clone Number
JE37-02
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
β2-glycoprotein 1, also known as beta-2 glycoprotein 1 and Apolipoprotein H (Apo-H), is a 38 kDa multifunctional plasma protein that in humans is encoded by the APOH gene. One of its functions is to bind cardiolipin. When bound, the structure of cardiolipin and β2-GP1 both undergo large changes in structure.[6] Within the structure of Apo-H is a stretch of positively charged amino acids (protein sequence positions 282-287), Lys-Asn-Lys-Glu-Lys-Lys, are involved in phospholipid binding (see image on right). β2-GP1 has a complex involvement in agglutination. It appears to alter adenosine diphosphate (ADP)-mediated agglutination of platelets. Normally, β2-GP1 assumes an anticoagulation activity in serum (by inhibiting coagulation factors); however, changes in blood factors can result in a reversal of that activity. Although previously referred to as apolipoprotein H, it is not present in appreciable quantities in the lipoprotein fractions, so ApoH is therefore thought to be a misnomer.
Background References
1. Tan Y et al. Exosome-Contained APOH Associated With Antiphospholipid Syndrome. Front Immunol. 2021 May
2. Lu Y et al. Analysis of the Relationship between the Expression Level of TTR and APOH and Prognosis in Patients with Colorectal Cancer Metastasis Based on Bioinformatics. Contrast Media Mol Imaging. 2022 Sep
Subcellular Location
Secreted.
UNIPROT
Synonyms
Activated protein C binding protein antibody
Activated protein C-binding protein antibody
Anticardiolipin cofactor antibody
APC inhibitor antibody
Apo-H antibody
APOH antibody
APOH_HUMAN antibody
apolipoprotein H (beta-2-glycoprotein I) antibody
Apolipoprotein H antibody
B2G1 antibody
ExpandImages
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Western blot analysis of Apo-H on human liver tissue lysates with Rabbit anti-Apo-H antibody (HA721649) at 1/1,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38/55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721649) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Apo-H on different lysates with Rabbit anti-Apo-H antibody (HA721649) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD Apo-H cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"