WIF1 Recombinant Rabbit Monoclonal Antibody [JE41-39]
Catalog# HA721660
WIF1 Recombinant Rabbit Monoclonal Antibody [JE41-39]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
WIF1 Recombinant Rabbit Monoclonal Antibody [JE41-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human WIF1 aa 330-379 / 379.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
HT-29 cell lysate, HeLa cell lysate, SW480 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, mouse heart tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, human lung tissue lysate.
Conjugation
unconjugated
Clone Number
JE41-39
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. WIF1 is a lipid-binding protein that binds to Wnt proteins and prevents them from triggering signalling. WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
Background References
1. Sun H et al. Loss of CLDN5 in podocytes deregulates WIF1 to activate WNT signaling and contributes to kidney disease. Nat Commun. 2022 Mar
2. Wei Y et al. miR-424-5p shuttled by bone marrow stem cells-derived exosomes attenuates osteogenesis via regulating WIF1-mediated Wnt/β-catenin axis. Aging (Albany NY). 2021 Jul
Subcellular Location
Secreted.
Synonyms
WIF-1 antibody
wif1 antibody
WIF1_HUMAN antibody
Wnt inhibitory factor 1 antibody
Images
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Western blot analysis of WIF1 on different lysates with Rabbit anti-WIF1 antibody (HA721660) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: SW480 cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Mouse heart tissue lysate (40 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)
Lane 8: Rat lung tissue lysate (40 µg/Lane)
Lane 9: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"