TCF-4 Recombinant Rabbit Monoclonal Antibody [JE41-73] (HA721664)
Catalog# HA721664
Rabbit Monoclonal to TCF-4
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TCF-4 on different lysates with Rabbit anti-TCF-4 antibody (HA721664) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Caco-2 cell lysate
Lane 5: SW620 cell lysate
Lane 6: HeLa cell lysate
Lane 7: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 55 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721664) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Caco-2 cell lysate
Lane 5: SW620 cell lysate
Lane 6: HeLa cell lysate
Lane 7: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 55 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721664) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
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