Phospho-PI3K p85+PI3K p55 (p85(pY467)+p55(pY199)) Recombinant Rabbit Monoclonal Antibody [PSH01-38] (HA721672)
Catalog# HA721672
Rabbit Monoclonal to Phospho-PI3K p85+PI3K p55 (p85(pY467)+p55(pY199))
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WB
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IF-Cell
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Human
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Mouse
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 55/85 kDa
Exposure time: 3 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721672) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-PI3K p85+PI3K p55 (p85(pY467)+p55(pY199)) Recombinant Rabbit Monoclonal Antibody [PSH01-38] (HA721672)
Immunogen
Synthetic peptide within human pik3r1 aa 451-500 / 724.
Host
Rabbit
Modification
Phospho
Modification Site
p85(pY467)+p55(pY199)
Positive Control
NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, NIH/3T3 cells treated with 1mM Sodium orthovanadate for 30 minutes, 293T overexpress Src cell lysate, 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH01-38
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 84 kDa/55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-PI3K p85+PI3K p55 (p85(pY467)+p55(pY199))
SYNONYMS
GRB1 antibody;p85 alpha antibody;p85 antibody;P85A_HUMAN antibody;Phosphatidylinositol 3 kinase associated p 85 alpha antibody;Phosphatidylinositol 3 kinase regulatory 1 antibody;Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody;Phosphatidylinositol 3-kinase regulatory subunit alpha antibody;Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody;PI3 kinase p85 antibody;PI3 kinase p85 subunit alpha antibody;PI3-kinase regulatory subunit alpha antibody;PI3-kinase subunit p85-alpha antibody;PI3K antibody;PI3K p85 antibody;PI3K regulatory subunit alpha antibody;Pik3r1 antibody;PtdIns 3 kinase p85 alpha antibody;PtdIns-3-kinase regulatory subunit alpha antibody;PtdIns-3-kinase regulatory subunit p85-alpha antibody
SUBCELLULAR LOCATION
Cell-cell junction, cis-Golgi network, cytoplasm, cytosol, membrane, nucleus.
FUNCTION
Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
CITATIONS
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Rao Zichen
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WB
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Human
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Lactoferrin alleviates acute alcoholic liver injury by improving redox-stress response capacity in female C57BL/6J mice
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