PSMB10 Recombinant Rabbit Monoclonal Antibody [PSH01-58]
Catalog# HA721701
PSMB10 Recombinant Rabbit Monoclonal Antibody [PSH01-58]
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WB
-
IHC-P
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Human
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Mouse
-
unconjugated
Overview
Product Name
PSMB10 Recombinant Rabbit Monoclonal Antibody [PSH01-58]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PSMB10 aa 1-273.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
HeLa treated with 100ng/mL IFN-γ for 48 hours cell lysate, HeLa cell lysate, Daudi cell lysate, Raji cell lysate, THP-1 cell lysate, Ramos cell lysate, U-937 cell lysate, HL-60 cell lysate, Jurkat cell lysate, SiHa cell lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, human lung tissue.
Conjugation
unconjugated
Clone Number
PSH01-58
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Proteasome subunit beta type-10 as known as 20S proteasome subunit beta-2i is a protein that in humans is encoded by the PSMB10 gene. This protein has a major role in the immune system as part of an immunoproteasome that is primarily induced upon infection and formed by replacing constitutive beta subunits with inducible beta subunits which possess specific cleavage properties that aid in the release of peptides necessary for MHC class I antigen presentation. The immunoproteasome appears to have a pivotal role in modulating NFκB signaling. The proteasome and its subunits are of clinical significance for at least two reasons: (1) a compromised complex assembly or a dysfunctional proteasome can be associated with the underlying pathophysiology of specific diseases, and (2) they can be exploited as drug targets for therapeutic interventions. The proteasomes form a pivotal component for the ubiquitin–proteasome system (UPS) and corresponding cellular Protein Quality Control (PQC). Protein ubiquitination and subsequent proteolysis and degradation by the proteasome are important mechanisms in the regulation of the cell cycle, cell growth and differentiation, gene transcription, signal transduction and apoptosis. During the antigen processing for the major histocompatibility complex (MHC) class-I, the proteasome is the major degradation machinery that degrades the antigen and present the resulting peptides to cytotoxic T lymphocytes. The immunoproteasome has been considered playing a critical role in improving the quality and quantity of generated class-I ligands.
Background References
1. Larsen F, Solheim J, Kristensen T, Kolstø AB, Prydz H (Oct 1993). "A tight cluster of five unrelated human genes on chromosome 16q22.1". Human Molecular Genetics. 2 (10): 1589–95.
2. Sulistio YA, Heese K (Jan 2015). "The Ubiquitin-Proteasome System and Molecular Chaperone Deregulation in Alzheimer\'s Disease". Molecular Neurobiology. 53 (2): 905–31.
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
beta2i antibody
FLJ00366 antibody
LMP10 antibody
Low molecular mass protein 10 antibody
Macropain subunit MECl 1 antibody
Macropain subunit MECl-1 antibody
MECL1 antibody
MGC1665 antibody
Multicatalytic endopeptidase complex subunit MECl 1 antibody
Multicatalytic endopeptidase complex subunit MECl-1 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of PSMB10 on different lysates with Rabbit anti-PSMB10 antibody (HA721701) at 1/1,000 dilution.
Lane 1: HeLa treated with 100ng/mL IFN-γ for 48 hours cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Daudi cell lysate (20 µg/Lane)
Lane 4: Raji cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: Ramos cell lysate (20 µg/Lane)
Lane 7: U-937 cell lysate (20 µg/Lane)
Lane 8: HL-60 cell lysate (20 µg/Lane)
Lane 9: Jurkat cell lysate (20 µg/Lane)
Lane 10: SiHa cell lysate (20 µg/Lane)
Lane 11: Mouse spleen tissue lysate (40 µg/Lane)
Lane 12: Mouse thymus tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 25/29 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721701) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10 antibody (HA721701) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"