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AP-2 complex subunit alpha-1 Recombinant Rabbit Monoclonal Antibody [JE40-38]

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Specification

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Catalog# HA721717

AP-2 complex subunit alpha-1 Recombinant Rabbit Monoclonal Antibody [JE40-38]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

AP-2 complex subunit alpha-1 Recombinant Rabbit Monoclonal Antibody [JE40-38]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human AP-2 complex subunit alpha-1 aa 1-100 / 977.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 108 kDa

Positive Control

Mouse brain tissue lysate, rat brain tissue lysate, human brain tissue.

Conjugation

unconjugated

Clone Number

JE40-38

RRID

AB_3072830

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

Background References

1. Dam DHM et al. Flotillin and AP2A1/2 Promote IGF-1 Receptor Association with Clathrin and Internalization in Primary Human Keratinocytes. J Invest Dermatol. 2020 Sep

2. Adamopoulos PG et al. Molecular cloning of novel transcripts of the adaptor-related protein complex 2 alpha 1 subunit (AP2A1) gene, using Next-Generation Sequencing. Gene. 2018 Dec

Subcellular Location

Cell membrane, Membrane, coated pit.

UNIPROT

SwissProt: O95782 Human

SwissProt: P17426 Mouse

Entrez Gene: 308578 Rat

Synonyms

100 kDa coated vesicle protein A antibody

Adapter-related protein complex 2 alpha-1 subunit antibody

adapter-related protein complex 2 subunit alpha-1 antibody

Adaptin alpha A antibody

Adaptin alpha antibody

Adaptor protein complex AP 2 alpha 1 subunit antibody

adaptor protein complex AP 2 subunit alpha 1 antibody

Adaptor protein complex AP-2 subunit alpha-1 antibody

Adaptor protein complex AP2 alpha 1 subunit antibody

adaptor protein complex AP2 subunit alpha 1 antibody

Expand

Images

  • Western blot analysis of AP-2 complex subunit alpha-1 on different lysates with Rabbit anti-AP-2 complex subunit alpha-1 antibody (<a href="/products/HA721717" style="font-weight: bold;text-decoration: underline;">HA721717</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 108 kDa<br />Observed band size: 108 kDa<br /><br />Exposure time: 10 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721717" style="font-weight: bold;text-decoration: underline;">HA721717</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AP-2 complex subunit alpha-1 on different lysates with Rabbit anti-AP-2 complex subunit alpha-1 antibody (HA721717) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa

    Exposure time: 10 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721717) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-AP-2 complex subunit alpha-1 antibody (<a href="/products/HA721717" style="font-weight: bold;text-decoration: underline;">HA721717</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721717" style="font-weight: bold;text-decoration: underline;">HA721717</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-AP-2 complex subunit alpha-1 antibody (HA721717) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721717) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation