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COL11A1 Recombinant Rabbit Monoclonal Antibody [JE40-84]

Usd: 385

Specification

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Catalog# HA721718

COL11A1 Recombinant Rabbit Monoclonal Antibody [JE40-84]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

COL11A1 Recombinant Rabbit Monoclonal Antibody [JE40-84]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human COL11A1 aa 501-600 / 1,806.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 181 kDa

Positive Control

K-562 cell lysate, Jurkat cell lysate, PANC-1 cell lysate, HCT 116 cell lysate.

Conjugation

unconjugated

Clone Number

JE40-84

RRID

AB_3072831

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene.

Background References

1. Shi W et. al. COL11A1 as an novel biomarker for breast cancer with machine learning and immunohistochemistry validation. Front Immunol. 2022 Oct

2. Wu YH et. al. COL11A1 activates cancer-associated fibroblasts by modulating TGF-β3 through the NF-κB/IGFBP2 axis in ovarian cancer cells. Oncogene. 2021 Jul

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: P12107 Human

Synonyms

COBA1_HUMAN antibody

COL11A1 antibody

COLL6 antibody

Collagen alpha 1 antibody

Collagen alpha-1(XI) chain antibody

collagen XI alpha 1 antibody

collagen XI, alpha 1 polypeptide antibody

collagen, type XI, alpha 1 antibody

STL2 antibody

STL3 antibody

Expand

Images

  • Western blot analysis of COL11A1 on different lysates with Rabbit anti-COL11A1 antibody (<a href="/products/HA721718" style="font-weight: bold;text-decoration: underline;">HA721718</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: PANC-1 cell lysate<br />Lane 4: HCT 116 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 181 kDa<br />Observed band size: 130 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721718" style="font-weight: bold;text-decoration: underline;">HA721718</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of COL11A1 on different lysates with Rabbit anti-COL11A1 antibody (HA721718) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: PANC-1 cell lysate
    Lane 4: HCT 116 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 181 kDa
    Observed band size: 130 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721718) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation