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SNAP29 Recombinant Rabbit Monoclonal Antibody [PSH01-76]

Usd: 385

Specification

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Catalog# HA721728

SNAP29 Recombinant Rabbit Monoclonal Antibody [PSH01-76]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

SNAP29 Recombinant Rabbit Monoclonal Antibody [PSH01-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SNAP29 aa 1-258 / 258.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 29 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, U-87 MG cell lysate.

Conjugation

unconjugated

Clone Number

PSH01-76

RRID

AB_3072840

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.

Background References

1. Hou P, et al. 2023. The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.Autophagy. 19(2):551-569.

2. Zhou F, et al. 2018. Down-regulation of OGT promotes cisplatin resistance by inducing autophagy in ovarian cancer. Theranostics. 8(19):5200-5212.

Subcellular Location

Cytoplasm, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Cell projection, cilium membrane.

UNIPROT

SwissProt:O95721 Human

Synonyms

CEDNIK antibody

FLJ21051 antibody

SNAP 29 antibody

SNAP-29 antibody

SNAP29 antibody

SNP29_HUMAN antibody

Soluble 29 kDa NSF attachment protein antibody

Synaptosomal associated protein 29 antibody

Synaptosomal associated protein 29kDa antibody

Synaptosomal-associated protein 29 antibody

Expand

Images

  • Western blot analysis of SNAP29 on different lysates with Rabbit anti-SNAP29 antibody (<a href="/products/HA721728" style="font-weight: bold;text-decoration: underline;">HA721728</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: A549 cell lysate<br />Lane 5: U-87 MG cell lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29 kDa<br /><br />Exposure time: 5 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721728" style="font-weight: bold;text-decoration: underline;">HA721728</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SNAP29 on different lysates with Rabbit anti-SNAP29 antibody (HA721728) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: A549 cell lysate
    Lane 5: U-87 MG cell lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: 5 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721728) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"