MBD1 Recombinant Rabbit Monoclonal Antibody [PSH01-79]
Catalog# HA721731
MBD1 Recombinant Rabbit Monoclonal Antibody [PSH01-79]
-
WB
-
IF-Cell
-
FC
-
Mouse
-
Rat
-
HA750753
不含抗保成分
-
unconjugated
Overview
Product Name
MBD1 Recombinant Rabbit Monoclonal Antibody [PSH01-79]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse MBD1 aa 1-200 / 636.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 70 kDa
Positive Control
NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, mouse kidney tissue lysate, mouse brain tissue lysate, mouse lung tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, NIH/3T3.
Conjugation
unconjugated
Clone Number
PSH01-79
RRID
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Mouse |
|
|
|
| Rat |
|
||
| Human |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IF-Cell
-
1:100
-
FC
-
1:1,000
Target
Function
MBD1 is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Background References
1. Wensheng L, Bo Z, Qiangsheng H, et al. MBD1 promotes the malignant behavior of gallbladder cancer cells and induces chemotherapeutic resistance to gemcitabine. Cancer Cell Int. 2019 Sep 9; 19:232.
2. Lyst MJ, Nan X, Stancheva I. Retraction: Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. EMBO J. 2019 Oct 15;38(20): e103220.
Subcellular Location
Nucleus, Nucleus matrix, Nucleus speckle, Chromosome
Synonyms
CXXC 3 antibody
CXXC-type zinc finger protein 3 antibody
CXXC3 antibody
MBD 1 antibody
Mbd1 antibody
MBD1_HUMAN antibody
MECP1 COMPLEX antibody
Methyl CpG binding domain protein 1 antibody
Methyl CpG binding domain protein 1 isoform PCM1 antibody
Methyl CpG binding protein MBD1 antibody
ExpandImages
-
Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (HA721731) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: RAW264.7 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse lung tissue lysate
Lane 7: Mouse spleen tissue lysate
Lane 8: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721731) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NIH/3T3 cells labeling MBD1 with Rabbit anti-MBD1 antibody (HA721731) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MBD1 antibody (HA721731) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of NIH/3T3 cells labeling MBD1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721731, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"