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Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]

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Specification

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Catalog# HA721737

Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr183 of human MST1 protein.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 56 kDa

Positive Control

Ramos treated with 1μM Staurosporine for 1 hour cell lysate, NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate, C6 treated with 1μM Staurosporine for 3 hour cell lysate.

Conjugation

unconjugated

Clone Number

JE46-64

RRID

AB_3072849

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.

Background References

1. Liu M et al. Macrophage MST1/2 Disruption Impairs Post-Infarction Cardiac Repair via LTB4. Circ Res. 2021 Oct

2. Ueda Y et al. MST1/2 Balance Immune Activation and Tolerance by Orchestrating Adhesion, Transcription, and Organelle Dynamics in Lymphocytes. Front Immunol. 2020 May

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q13043 Human

SwissProt: Q13188 Human

SwissProt: Q9JI11 Mouse

SwissProt: Q9JI10 Mouse

SwissProt: O54748 Rat

Entrez Gene: 65189 Rat

Synonyms

DKFZp686A2068 antibody

FLJ90748 antibody

KRS1 antibody

KRS2 antibody

Mammalian STE20 like protein kinase 1 antibody

Mammalian STE20 like protein kinase 2 antibody

MST 1 antibody

MST 2 antibody

MST1 antibody

MST2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (<a href="/products/HA721737" style="font-weight: bold;text-decoration: underline;">HA721737</a>) at 1/1,000 dilution.<br /><br />Lane 1: Ramos cell lysate<br />Lane 2: Ramos treated with 1μM Staurosporine for 1 hour cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721737" style="font-weight: bold;text-decoration: underline;">HA721737</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (HA721737) at 1/1,000 dilution.

    Lane 1: Ramos cell lysate
    Lane 2: Ramos treated with 1μM Staurosporine for 1 hour cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721737) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (<a href="/products/HA721737" style="font-weight: bold;text-decoration: underline;">HA721737</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: C6 treated with 1μM Staurosporine for 3 hour cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 2 minutes 18 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721737" style="font-weight: bold;text-decoration: underline;">HA721737</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-MST1/MST2 (T180 + T183) on different lysates with Rabbit anti-Phospho-MST1/MST2 (T180 + T183) antibody (HA721737) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate
    Lane 3: C6 cell lysate
    Lane 4: C6 treated with 1μM Staurosporine for 3 hour cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

    Exposure time: 2 minutes 18 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721737) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation