Skip to content

MICU2 Recombinant Rabbit Monoclonal Antibody [PSH01-84]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721742

MICU2 Recombinant Rabbit Monoclonal Antibody [PSH01-84]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

MICU2 Recombinant Rabbit Monoclonal Antibody [PSH01-84]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human MICU2 aa 23-434.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, FC

Molecular Weight

Predicted band size: 50 kDa

Positive Control

LNCaP cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human kidney tissue lysate, human liver tissue lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human heart tissue, human kidney tissue, human liver tissue, mouse heart tissue, rat kidney tissue, LNCaP.

Conjugation

unconjugated

Clone Number

PSH01-84

RRID

AB_3072854

Reactivity Data

WB IHC-P IF-Tissue FC
Human
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Not Recommended
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • IF-Tissue

  • 1:200

  • FC

  • 1:1,000

Target

Function

Enables protein heterodimerization activity. Involved in calcium import into the mitochondrion and negative regulation of mitochondrial calcium ion concentration. Located in mitochondrial inner membrane and mitochondrial intermembrane space. Part of uniplex complex. Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low. MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium. MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting calcium, avoiding energy dissipation and cell-death triggering.

Background References

1. Patron M, Checchetto V, Raffaello A, et al. MICU1 and MICU2 finely tune the mitochondrial Ca2+ uniporter by exerting opposite effects on MCU activity. Mol Cell. 2014 Mar 6;53(5):726-37.

2. Matesanz-Isabel J, Arias-del-Val J, Alvarez-Illera P, et al. Functional roles of MICU1 and MICU2 in mitochondrial Ca (2+) uptake. Biochim Biophys Acta. 2016 Jun;1858(6):1110-7.

Subcellular Location

Mitochondrion

UNIPROT

SwissProt: Q8IYU8 Human

SwissProt: Q8CD10 Mouse

SwissProt: Q99P63 Rat

Synonyms

1110008L20Rik antibody

EF hand domain containing family member A1 antibody

EF hand domain family A1 antibody

EF hand domain family member A1 antibody

EF-hand domain-containing family member A1 antibody

EFHA1 antibody

EFHA1 EF hand domain family member A1 antibody

EFHA1_HUMAN antibody

FLJ25016 antibody

FLJ34588 antibody

Expand

Images

  • Western blot analysis of MICU2 on different lysates with Rabbit anti-MICU2 antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution.<br /><br />Lane 1: LNCaP cell lysate, 30 µg/Lane.<br />Lane 2: NIH/3T3 cell lysate, 30 µg/Lane.<br />Lane 3: PC-12 cell lysate, 30 µg/Lane.<br />Lane 4: Human kidney tissue lysate, 30 µg/Lane.<br />Lane 5: Human liver tissue lysate, 30 µg/Lane.<br />Lane 6: Mouse heart tissue lysate, 30 µg/Lane.<br />Lane 7: Mouse kidney tissue lysate, 30 µg/Lane.<br />Lane 8: Rat kidney tissue lysate, 30 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 42 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MICU2 on different lysates with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution.

    Lane 1: LNCaP cell lysate, 30 µg/Lane.
    Lane 2: NIH/3T3 cell lysate, 30 µg/Lane.
    Lane 3: PC-12 cell lysate, 30 µg/Lane.
    Lane 4: Human kidney tissue lysate, 30 µg/Lane.
    Lane 5: Human liver tissue lysate, 30 µg/Lane.
    Lane 6: Mouse heart tissue lysate, 30 µg/Lane.
    Lane 7: Mouse kidney tissue lysate, 30 µg/Lane.
    Lane 8: Rat kidney tissue lysate, 30 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 42 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721742) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-MICU2 antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MICU2 antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MICU2 antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MICU2 antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-MICU2 antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721742) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded human heart tissue labeling MICU2 with Rabbit anti-MICU2 antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human heart tissue labeling MICU2 with Rabbit anti-MICU2 antibody (HA721742) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721742, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Flow cytometric analysis of LNCaP cells labeling MICU2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721742" style="font-weight: bold;text-decoration: underline;">HA721742</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of LNCaP cells labeling MICU2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721742, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"