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SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-91]

Usd: 385

Specification

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Catalog# HA721749

SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-91]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

SHARP2 Recombinant Rabbit Monoclonal Antibody [PSH01-91]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SHARP2 aa 151-412 / 412.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 46 kDa

Positive Control

MCF7 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, A431 cell lysate.

Conjugation

unconjugated

Clone Number

PSH01-91

RRID

AB_3072861

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation.

Background References

1. Wang L et al. Single-cell RNA-seq analysis reveals BHLHE40-driven pro-tumour neutrophils with hyperactivated glycolysis in pancreatic tumour microenvironment. Gut. 2023 May

2. Zhang Y et al. BHLHE40 promotes osteoclastogenesis and abnormal bone resorption via c-Fos/NFATc1. Cell Biosci. 2022 May

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: O14503 Human

Synonyms

BHE40_HUMAN antibody

bHLHB2 antibody

bHLHe40 antibody

Class B Basic Helix Loop Helix Protein 2 antibody

Class B basic helix-loop-helix protein 2 antibody

Class E basic helix loop helix protein 40 antibody

Class E basic helix-loop-helix protein 40 antibody

Clast5 antibody

DEC1 antibody

Differentially expressed in chondrocytes protein 1 antibody

Expand

Images

  • Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (<a href="/products/HA721749" style="font-weight: bold;text-decoration: underline;">HA721749</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: MDA-MB-231 cell lysate<br />Lane 4: 293T cell lysate<br />Lane 5: A431 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 46 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721749" style="font-weight: bold;text-decoration: underline;">HA721749</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SHARP2 on different lysates with Rabbit anti-SHARP2 antibody (HA721749) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: MDA-MB-231 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: A431 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"