Cre recombinase Recombinant Rabbit Monoclonal Antibody [PSH01-93] (HA721751)
Catalog# HA721751
Rabbit Monoclonal to Cre recombinase
Tested for
-
WB
-
IHC-P
-
IF-Tissue
-
IF-Cell
-
FC
Reacts with
-
Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Cre recombinase on different lysates with Rabbit anti-Cre recombinase antibody (HA721751) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with Cre recombinase cell lysate
Lysates/proteins at 50 ng/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721751) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with Cre recombinase cell lysate
Lysates/proteins at 50 ng/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721751) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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