FLIP Recombinant Rabbit Monoclonal Antibody [PSH01-95] (HA721753)
Catalog# HA721753
Rabbit Monoclonal to FLIP
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of FLIP on different lysates with Rabbit anti-FLIP antibody (HA721753) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: HeLa cell lysate
Lane 3: 293T cell lysate
Lane 4: K-562 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: MDA-MB-468 cell lysate
Lane 7: MDA-MB-231 cell lysate
Lane 8: Jurkat cell lysate
Lane 9: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55/25 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721753) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: HeLa cell lysate
Lane 3: 293T cell lysate
Lane 4: K-562 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: MDA-MB-468 cell lysate
Lane 7: MDA-MB-231 cell lysate
Lane 8: Jurkat cell lysate
Lane 9: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55/25 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721753) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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