Mouse LIF Recombinant Rabbit Monoclonal Antibody [PSH02-00] - BSA and Azide free (Capture)
Overview
Product Name
Mouse LIF Recombinant Rabbit Monoclonal Antibody [PSH02-00] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse LIF aa 24-203 (P09056).
Species Reactivity
Mouse
Validated Applications
ELISA(Cap)
Molecular Weight
Predicted band size: 22.3 kDa
Positive Control
Recombinant mouse Lif protein (HA210735).
Conjugation
unconjugated
Clone Number
PSH02-00
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-01] to Mouse LIF (Detector) (HA721759) and recombinant standard Mouse LIF (HA210735) as the standard. The reference range value is 8.2-2,000 pg/mL.
Target
Function
The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Background References
暂无
Subcellular Location
Secreted
UNIPROT
Synonyms
CDF antibody
Cholinergic Differentiation Factor antibody
D factor antibody
DIA antibody
Differentiation inducing factor antibody
differentiation inhibitory activity antibody
Differentiation stimulating factor antibody
Differentiation-stimulating factor antibody
Emfilermin antibody
Hepatocyte stimulating factor III antibody
ExpandImages
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![Sandwich ELISA analysis of mouse Lif matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721758" style="font-weight: bold;text-decoration: underline;">HA721758</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse Lif protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-01]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721758_1.jpg?v=20260429161654)
Sandwich ELISA analysis of mouse Lif matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721758) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse Lif protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-01]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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